Characterization of Intracellular Ca 2+ Handling in Vascular Smooth Muscle Cells Isolated from Amputated Human Limbs
| العنوان: | Characterization of Intracellular Ca 2+ Handling in Vascular Smooth Muscle Cells Isolated from Amputated Human Limbs |
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| المؤلفون: | Rebecca Bruning, Michael Sturek |
| المصدر: | The FASEB Journal. 29 |
| بيانات النشر: | Wiley, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | medicine.medical_specialty, Vascular smooth muscle, business.industry, Calcium handling, Endoplasmic reticulum, medicine.medical_treatment, Anatomy, musculoskeletal system, Biochemistry, Peripheral, chemistry.chemical_compound, Endocrinology, chemistry, Amputation, Internal medicine, Genetics, Extracellular, medicine, Caffeine, business, Molecular Biology, Intracellular, Biotechnology |
| الوصف: | High Ca2+ scores assessed by computed tomography in the lower limbs of patients with peripheral artery disease are associated with a greater risk of amputation and all-cause mortality. Direct assessment of intracellular Ca2+ handling in peripheral vascular smooth muscle cells (VSM) of humans likely to undergo an amputation is unknown due to the invasiveness of such measures. We directly characterized intracellular VSM Ca2+ handling in arteries from amputated human limbs stratified by the number of diagnosed cardiovascular (CV) risk factors ( 6 CV risk factors). VSM were enzymatically isolated from peripheral arteries and were digitally imaged using the fluorescent intracellular Ca2+ indicator, fura-2. Cells were exposed to 80 mM K+ in physiologic Ca2+ to assess Ca2+ entry through voltage-gated Ca2+ channels (VGCC). Caffeine was used in the presence and absence of extracellular Ca2+ to release intracellular Ca2+ from the sarcoplasmic reticulum (SR). VSM from patients with >6 CV risk factors had eleva... |
| تدمد: | 1530-6860 0892-6638 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::3462bfd042d62a02c43aa0d13a9be633Test https://doi.org/10.1096/fasebj.29.1_supplement.638.3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........3462bfd042d62a02c43aa0d13a9be633 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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