Next-generation sequencing not superior to culture in periprosthetic joint infection diagnosis
| العنوان: | Next-generation sequencing not superior to culture in periprosthetic joint infection diagnosis |
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| المؤلفون: | Alexander L. Lazarides, Richard Danilkowicz, Colin T. Penrose, Michael P. Bolognesi, William A. Jiranek, Beau J. Kildow, Thorsten M. Seyler, Sean P. Ryan |
| المصدر: | The Bone & Joint Journal. :26-31 |
| بيانات النشر: | British Editorial Society of Bone & Joint Surgery, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, Prosthesis-Related Infections, Periprosthetic, Computational biology, Polymerase Chain Reaction, Sensitivity and Specificity, DNA sequencing, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Humans, Medicine, Orthopedics and Sports Medicine, 030212 general & internal medicine, Polymerase chain reaction, Aged, Retrospective Studies, Aged, 80 and over, Bacteriological Techniques, 030222 orthopedics, business.industry, High-Throughput Nucleotide Sequencing, Infection diagnosis, Middle Aged, Reference Standards, Female, Surgery, Identification (biology), business |
| الوصف: | Aims Use of molecular sequencing methods in periprosthetic joint infection (PJI) diagnosis and organism identification have gained popularity. Next-generation sequencing (NGS) is a potentially powerful tool that is now commercially available. The purpose of this study was to compare the diagnostic accuracy of NGS, polymerase chain reaction (PCR), conventional culture, the Musculoskeletal Infection Society (MSIS) criteria, and the recently proposed criteria by Parvizi et al in the diagnosis of PJI. Methods In this retrospective study, aspirates or tissue samples were collected in 30 revision and 86 primary arthroplasties for routine diagnostic investigation for PJI and sent to the laboratory for NGS and PCR. Concordance along with statistical differences between diagnostic studies were calculated. Results Using the MSIS criteria to diagnose PJI as the reference standard, the sensitivity and specificity of NGS were 60.9% and 89.9%, respectively, while culture resulted in sensitivity of 76.9% and specificity of 95.3%. PCR had a low sensitivity of 18.4%. There was no significant difference based on sample collection method (tissue swab or synovial fluid) (p = 0.760). There were 11 samples that were culture-positive and NGS-negative, of which eight met MSIS criteria for diagnosing infection. Conclusion In our series, NGS did not provide superior sensitivity or specificity results compared to culture. PCR has little utility as a standalone test for PJI diagnosis with a sensitivity of only 18.4%. Currently, several laboratory tests for PJI diagnosis should be obtained along with the overall clinical picture to help guide decision-making for PJI treatment. Cite this article: Bone Joint J 2021;103-B(1):26–31. |
| تدمد: | 2049-4408 2049-4394 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27efc928845c9693e0e6a784999eb54bTest https://doi.org/10.1302/0301-620x.103b1.bjj-2020-0017.r3Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....27efc928845c9693e0e6a784999eb54b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20494408 20494394 |
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