Na + /Ca 2+ Exchanger Maintains Ionic Homeostasis in the Peri-Infarct Area
| العنوان: | Na + /Ca 2+ Exchanger Maintains Ionic Homeostasis in the Peri-Infarct Area |
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| المؤلفون: | Ilaria Barone, Giorgio Bernardi, Cinzia Costa, Anna Tortiglione, Alessandro Tozzi, Massimiliano Di Filippo, Silvia Rossi, Paolo Calabresi, Barbara Picconi, Michela Tantucci, Lucio Annunziato, Diego Centonze |
| المساهمون: | Tortiglione, A, Picconi, B, Barone, I, Centonze, D, Rossi, S, Costa, C, Di Filippo, M, Tozzi, A, Tantucci, M, Bernardi, G, Annunziato, Lucio, Calabresi, P., A., Tortiglione, B., Picconi, I., Barone, D., Centonze, S., Rossi, C., Costa, M., Di Filippo, A., Tozzi, M., Tantucci, G., Bernardi, P., Calabresi |
| المصدر: | Stroke. 38:1614-1620 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Male, Middle Cerebral Artery, striatum, Ischemia, ischemia, Sodium-Calcium Exchanger, Brain Ischemia, Rats, Sprague-Dawley, Brain ischemia, medicine.artery, medicine, Extracellular, Animals, Homeostasis, Patch clamp, Infarction, Middle Cerebral Artery, Ions, Rats, Advanced and Specialized Nursing, Na+/Ca2+ exchanger, business.industry, Anatomy, electrophysiology, medicine.disease, field potential, Electrophysiology, permanent middle cerebral artery occlusion, Infarction, Bepridil, Middle cerebral artery, Biophysics, Settore MED/26 - Neurologia, Sprague-Dawley, Neurology (clinical), Cardiology and Cardiovascular Medicine, business, medicine.drug |
| الوصف: | Background and Purpose— A prominent feature of cerebral ischemia is the excessive intracellular accumulation of both Na + and Ca 2+ ions, which results in subsequent cell death. The plasma membrane Na + /Ca 2+ exchanger (NCX), regulates the distribution of these ions acting either in the forward mode or in its reverse mode and it can play a critical role in brain ischemia. However, it is unclear whether the activity of NCX leads to detrimental or beneficial effects. Methods— Extracellular field potentials and whole-cell patch clamp recordings were obtained from rat corticostriatal brain-slice preparations in the peri-infarct area 24 hours after the permanent middle cerebral artery occlusion. Ischemia was induced in rats by permanents middle cerebral artery occlusion. Results— Bepridil, an inhibitor of NCX, reduced in a concentration-dependent manner (IC 50 =68 μmol/L) the field potential amplitude recorded from the peri-infarct area of corticostriatal slices. Conversely, no change was observed in sham-operated animals. The effect of bepridil was mimicked by 5-( N -4-chlorobenzyl)-2′,4′-dimethylbenzamil (CB-DMB) (IC 50 =6 μmol/L), a more selective inhibitor of NCX. In whole-cell patch clamp experiments, bepridil and CB-DMB caused an inward current in spiny neurons recorded from the peri-infarct area but not in the same cells recorded from controls. Interestingly, cholinergic interneurons recorded from the striatal peri-infarct area did not develop an inward current after the application of NCX inhibitors, suggesting that the electrophysiological alterations induced by NCX inhibition are cell-type specific. Bepridil and CB-DMB also induced a suppression of excitatory synaptic currents in most of spiny neurons recorded from the peri-infarct area. This effect was not coupled to a significant change of paired-pulse facilitation suggesting a postsynaptic site of action. Conclusions— Our data indicate that NCX plays a critical role in the maintenance of ionic homeostasis in the peri-infarct area. |
| وصف الملف: | STAMPA |
| تدمد: | 1524-4628 0039-2499 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cca741e73748314ee5052cdf002fa9aTest https://doi.org/10.1161/strokeaha.106.478644Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1cca741e73748314ee5052cdf002fa9a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244628 00392499 |
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