Isolation stress impacts Met-enkephalin in the hypothalamo-pituitary-adrenocortical axis in growing Polish Mountain sheep: a possible role of the opioids in modulation of HPA axis
| العنوان: | Isolation stress impacts Met-enkephalin in the hypothalamo-pituitary-adrenocortical axis in growing Polish Mountain sheep: a possible role of the opioids in modulation of HPA axis |
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| المؤلفون: | Colin G. Scanes, Krystyna Pierzchała-Koziec, Marta Dziedzicka-Wasylewska |
| المصدر: | Stress. 22:256-264 |
| بيانات النشر: | Informa UK Limited, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Met-enkephalin, Hypothalamo-Hypophyseal System, endocrine system, medicine.medical_specialty, Pituitary gland, Physiology, Enkephalin, Methionine, Hypothalamus, Pituitary-Adrenal System, isolation stress, Biology, Naltrexone, 03 medical and health sciences, Behavioral Neuroscience, Basal (phylogenetics), chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, tissue Met-enkephalin, Gene expression, medicine, Animals, hypothalamo-pituitary axis, Sheep, PENK mRNA, Endocrine and Autonomic Systems, Adrenal cortex, digestive, oral, and skin physiology, In vitro, 030227 psychiatry, Analgesics, Opioid, Psychiatry and Mental health, Neuropsychology and Physiological Psychology, Endocrinology, medicine.anatomical_structure, Social Isolation, nervous system, chemistry, Pituitary Gland, Female, Poland, Corticosterone, Stress, Psychological, hormones, hormone substitutes, and hormone antagonists, 030217 neurology & neurosurgery, in vitro enkephalin release, medicine.drug |
| الوصف: | It was hypothesized that there is cross-talk between the classical constituents of the hypothalamo-pituitary-adrenocortical axis (HPA) and Met-enkephalin in the HPA axis. The study examined effects of isolation stress, sex, and age on concentrations of native Met-enkephalin and pro-enkephalin (PENK) gene expression in tissues of the HPA (hypothalamus, pituitary gland and adrenal cortex) in 3-, 6- and 9-month old female and male lambs. In addition, the effects of isolation stress on in vitro release Met-enkephalin from fragments of the hypothalamus or adrenal cortex were examined. Isolation stress was followed by decreases in the concentration of Met-enkephalin in both the pituitary gland and adrenal cortex. There were also increases in the hypothalamic concentration of Met-enkephalin together with increases in PENK gene expression in the HPA in 6- and 9-months old females and males. There were reductions in release of Met-enkephalin from hypothalamic and adrenocortical tissue in vitro after isolation stress. In the presence of naltrexone, there were increases in basal release in vitro of Met-enkephalin from hypothalamic tissue from control and stressed female lambs but a decrease in tissue from stressed male lambs. In a somewhat similar manner, the presence of naltrexone was associated with increases in the basal release of Met-enkephalin from adrenocortical tissue from control female lambs but a decrease with tissue from stressed female and both stressed and control male lambs. Lay summary The present studies examine the impact of isolation stress on Met-enkephalin in growing female and male lambs. The results clearly showed the involvement of Met-enkephalin modulation of the psychological stress response in growing female and male lambs. |
| تدمد: | 1607-8888 1025-3890 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b444d295ce6776a1cfeb389e305cee5bTest https://doi.org/10.1080/10253890.2018.1553947Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b444d295ce6776a1cfeb389e305cee5b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16078888 10253890 |
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