Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells
| العنوان: | Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells |
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| المؤلفون: | Michael D'Amico, Elizabeth Cunningham, Michael Scarpone, Daniel Kuebler, Michael F. McLaughlin, Randi Yeager, Katie Guski, Paul Gagnet |
| المصدر: | Stem Cells International, Vol 2016 (2016) Stem Cells International |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Internal medicine, Article Subject, business.industry, Cell, Adipose tissue, Cell Biology, In vitro, Andrology, 03 medical and health sciences, 030104 developmental biology, Thrombin, medicine.anatomical_structure, Adipogenesis, Immunology, Gene expression, Medicine, Stem cell, lcsh:RC31-1245, business, Molecular Biology, Fetal bovine serum, Research Article, medicine.drug |
| الوصف: | The administration of human adipose-derived stem cells (ASCs) represents a promising regenerative therapy for the treatment of orthopedic injuries. While ASCs can be easily isolated from liposuction-derived adipose tissue, most clinical applications will likely requirein vitroculture expansion of these cells using nonxenogeneic components. In this study, platelet releasate was generated using a novel rapid thrombin activation method (tPR). ASCs grown in media supplemented with tPR proliferated much faster than ASCs grown in media supplemented with 10% fetal bovine serum. The cells also retained the ability to differentiate along chondrogenic, adipogenic, and osteogenic lineages. The tPR cultured ASCs displayed elevated expression of BMP-4 (5.7 ± 0.97-fold increase) and BMP-2 (4.7 ± 1.3-fold increase) and decreased expression of PDGF-B (4.0 ± 1.4-fold decrease) and FGF-2 (33 ± 9.0-fold decrease). No significant changes in expression were seen with TGF-βand VEGF. This pattern of gene expression was consistent across different allogeneic tPR samples and different ASC lines. The use of allogeneic rapidly activated tPR to culture ASCs is associated with both an increased cell yield and a defined gene expression profile making it an attractive option for cell expansion prior to cell-based therapy for orthopedic applications. |
| وصف الملف: | text/xhtml |
| تدمد: | 1687-9678 1687-966X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56dad9c502351fbd8302cb3651064dcdTest https://doi.org/10.1155/2016/7183734Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....56dad9c502351fbd8302cb3651064dcd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16879678 1687966X |
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