دورية أكاديمية
Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells
| العنوان: | Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells |
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| المؤلفون: | Peihua Zhang, Jin Li, Yawei Qi, Xudong Tang, Jianfeng Duan, Li Liu, Zeyong Wu, Jie Liang, Jiangfeng Li, Xian Wang, Guofang Zeng, Hongwei Liu |
| المصدر: | Stem Cells International, Vol 2016 (2016) |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Internal medicine |
| مصطلحات موضوعية: | Internal medicine, RC31-1245 |
| الوصف: | Tissue inhibitor of metalloproteinases-1 (TIMP-1) is a multifunctional matrix metalloproteinase, and it is involved in the regulation of cell proliferation and apoptosis in various cell types. However, little is known about the effect of TIMP-1 expression on the proliferation of adipose-derived stem cells (ADSCs). Therefore, TIMP-1 expression in the ADSCs was firstly detected by western blotting, and TIMP-1 gene was knocked down by lentivirus-mediated shRNA. Cell proliferation was then evaluated by MTT assay and Ki67 staining, respectively. Cell cycle progression was determined by flow cytometry. The changes of p51, p21, cyclin E, cyclin-dependent kinase 2 (CDK2), and P-CDK2 caused by TIMP-1 knockdown were detected by western blotting. The results indicated that ADSCs highly expressed TIMP-1 protein, and the knockdown of TIMP-1 inhibited cell proliferation and arrested cell cycle progression at G1 phase in the ADSCs possibly through the upregulation of p53, p21, and P-CDK2 protein levels and concurrent downregulation of cyclin E and CDK2 protein levels. These findings suggest that TIMP-1 works as a positive regulator of cell proliferation in ADSCs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1687-966X 1687-9678 |
| العلاقة: | https://doaj.org/toc/1687-966XTest; https://doaj.org/toc/1687-9678Test |
| DOI: | 10.1155/2016/4761507 |
| الوصول الحر: | https://doaj.org/article/69e3683105534e469b7513d5b969e040Test |
| رقم الانضمام: | edsdoj.69e3683105534e469b7513d5b969e040 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1687966X 16879678 |
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| DOI: | 10.1155/2016/4761507 |