p38 mitogen activated protein kinase controls two successive-steps during the early mesodermal commitment of embryonic stem cells.: P38 CONTROLS THE MESODERMAL COMMITMENT OF ES CELLS
| العنوان: | p38 mitogen activated protein kinase controls two successive-steps during the early mesodermal commitment of embryonic stem cells.: P38 CONTROLS THE MESODERMAL COMMITMENT OF ES CELLS |
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| المؤلفون: | Yasmine Hadjal, Ola Hadadeh, Didier Nègre, Valérie M. Renault, Emilie Barruet, Irène Juhan-Vague, Roselyne Tournaire, Bernard Binétruy, Denis Bernot, Marie-Christine Alessi, Franck Peiretti |
| المساهمون: | Fibrinolyse et Pathologie Vasculaire, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Stress Cellulaire, IFR128, Virologie humaine, École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by a grant from the Association Française contre les Myopathies. E. B. was supported by fellowships from the Groupe d'Eude sur l'Hémostase et la Thrombose and from the Fondation pour la Recherche Médicale O. H. was supported by a fellowship from the Association Française contre les Myopathies., École normale supérieure - Lyon (ENS Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), TOURNAIRE, ROSELYNE |
| المصدر: | Stem Cells and Development Stem Cells and Development, 2011, 20 (7), pp.1233-1246. ⟨10.1089/scd.2010.0213⟩ Stem Cells and Development, 2011, 20 (7), pp.1233-46. ⟨10.1089/scd.2010.0213⟩ |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Fetal Proteins, Cellular differentiation, Cell, MESH: Myocytes, Cardiac, Fluorescent Antibody Technique, MESH: Flow Cytometry, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Muscle Development, MESH: Mitogen-Activated Protein Kinase 14, Mesoderm, Mitogen-Activated Protein Kinase 14, Gene Knockout Techniques, Mice, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Myocyte, MESH: Animals, Myocytes, Cardiac, MESH: Embryonic Stem Cells, MESH: Fluorescent Antibody Technique, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Cells, Cultured, MESH: Gene Knockout Techniques, Regulation of gene expression, MESH: Mesoderm, MESH: Fetal Proteins, Neurogenesis, Imidazoles, Cell Differentiation, Hematology, Flow Cytometry, MESH: Gene Expression Regulation, Cell biology, medicine.anatomical_structure, MESH: Muscle Development, MESH: Imidazoles, MESH: Cells, Cultured, MESH: Cell Differentiation, Brachyury, MESH: T-Box Domain Proteins, Blotting, Western, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine, MESH: Blotting, Western, Animals, MESH: Mice, Embryonic Stem Cells, Skeletal muscle, Cell Biology, Embryonic stem cell, Gene Expression Regulation, Immunology, T-Box Domain Proteins, Developmental Biology |
| الوصف: | International audience; Embryonic stem (ES) cells differentiate in vitro into all cell lineages. We previously found that the p38 mitogen activated kinase (p38MAPK) pathway controls the commitment of ES cells toward either cardiomyogenesis (p38 on) or neurogenesis (p38 off ). In this study, we show that p38α knock-out ES cells do not differentiate into cardiac, endothelial, smooth muscle, and skeletal muscle lineages. Reexpression of p38MAPK in these cells partially rescues their mesodermal differentiation defects and corrects the high level of spontaneous neurogenesis of knock-out cells. Wild-type ES cells were treated with a p38MAPK-specific inhibitor during the differentiation process. These experiments allowed us to identify 2 early independent successive p38MAPK functions in the formation of mesodermal lineages. Further, the first one correlates with the regulation of the expression of Brachyury, an essential mesodermal-specific transcription factor, by p38MAPK. In conclusion, by genetic and biochemical approaches, we demonstrate that p38MAPK activity is essential for the commitment of ES cell into cardiac, endothelial, smooth muscle, and skeletal muscle mesodermal lineages. |
| تدمد: | 1557-8534 1547-3287 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5cb159b4b52862649b48001acb469c6bTest https://pubmed.ncbi.nlm.nih.gov/20954847Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5cb159b4b52862649b48001acb469c6b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15578534 15473287 |
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