Mesenchymal stem cell-loaded porous tantalum integrated with biomimetic 3D collagen-based scaffold to repair large osteochondral defects in goats
| العنوان: | Mesenchymal stem cell-loaded porous tantalum integrated with biomimetic 3D collagen-based scaffold to repair large osteochondral defects in goats |
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| المؤلفون: | Hui Xie, Xiaowei Wei, Dewei Zhao, Lu Li, Xiaojie Dou, Zihua Wang, Yu Zhang, Guoshuang Zheng, Jiahui Yang, Ge Liu, Baoyi Liu, Zhijie Ma, Benjie Wang, Fang Cao, William C. Lineaweaver, Fan Yang, Liangliang Cheng, Daping Cui, Weiting Yu, Junlei Li, Wei Wang, Feng Zhang |
| المصدر: | Stem Cell Research & Therapy Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-17 (2019) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cartilage, Articular, Scaffold, Bone Regeneration, Biocompatibility, Medicine (miscellaneous), 3D collagen-based scaffold, Tantalum, Mesenchymal Stem Cell Transplantation, Osteochondral regeneration, Biochemistry, Genetics and Molecular Biology (miscellaneous), Fibrin, lcsh:Biochemistry, 03 medical and health sciences, Bone marrow mesenchymal stem cells, 0302 clinical medicine, Chondrocytes, Tissue engineering, Osteochondral defect, In vivo, Biomimetic Materials, Porous tantalum, Animals, lcsh:QD415-436, lcsh:R5-920, biology, Tissue Scaffolds, Chemistry, Goats, Research, Mesenchymal stem cell, Membranes, Artificial, Mesenchymal Stem Cells, Cell Biology, Chondrogenesis, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, Molecular Medicine, Collagen, Stem cell, lcsh:Medicine (General), Porosity, Biomedical engineering |
| الوصف: | Background The body is unable to repair and regenerate large area bone defects. Moreover, the repair capacity of articular cartilage is very limited. There has long been a lack of effective treatments for osteochondral lesions. The engineered tissue with biphase synthetic for osteochondral repair has become one of the hot research fields over the past few years. In this study, an integrated biomanufacturing platform was constructed with bone marrow mesenchymal stem cells (BMSCs)/porous tantalum (pTa) associated with chondrocytes/collagen membranes (CM) to repair large osteochondral defects in load-bearing areas of goats. Methods Twenty-four goats with a large osteochondral defect in the femoral heads of the left hind legs were randomly divided into three groups: eight were treated with chondrocytes/CM-BMSCs/pTa, eight were treated with pure CM-pTa composite, and the other eight goats were untreated. The repair effect was assessed by X-ray, gross observation, and histomorphology for 16 weeks after the operation. In addition, the biocompatibility of chondrocytes/CM-BMSCs/pTa was observed by flow cytometry, CCK8, immunocytochemistry, and Q-PCR. The characteristics of the chondrocytes/CM-BMSCs/pTa were evaluated using both scanning electron microscopy and mechanical testing machine. Results The integrated repair material consists of pTa, injectable fibrin sealant, and CM promoted adhesion and growth of BMSCs and chondrocytes. pTa played an important role in promoting the differentiation of BMSCs into osteoblasts. Three-dimensional CM maintained the phenotype of chondrocytes successfully and expressed chondrogenic genes highly. The in vivo study showed that after 16 weeks from implantation, osteochondral defects in almost half of the femoral heads had been successfully repaired by BMSC-loaded pTa associated with biomimetic 3D collagen-based scaffold. Conclusions The chondrocytes/CM-BMSCs/pTa demonstrated significant therapeutic efficacy in goat models of large osteochondral defect. This provides a novel therapeutic strategy for large osteochondral lesions in load-bearing areas caused by severe injury, necrosis, infection, degeneration, and tumor resection with a high profile of safety, effectiveness, and simplicity. |
| تدمد: | 1757-6512 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2b207727e053427be6f87ac1bcae113Test https://pubmed.ncbi.nlm.nih.gov/30837004Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e2b207727e053427be6f87ac1bcae113 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17576512 |
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