دورية أكاديمية
Human mesenchymal stem cells improve rat islet functionality under cytokine stress with combined upregulation of heme oxygenase-1 and ferritin
| العنوان: | Human mesenchymal stem cells improve rat islet functionality under cytokine stress with combined upregulation of heme oxygenase-1 and ferritin |
|---|---|
| المؤلفون: | Camille Laporte, Emily Tubbs, Justine Cristante, Anne-Sophie Gauchez, Sandra Pesenti, Frédéric Lamarche, Cécile Cottet-Rousselle, Catherine Garrel, Anaick Moisan, Jean-Marc Moulis, Eric Fontaine, Pierre-Yves Benhamou, Sandrine Lablanche |
| المصدر: | Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-12 (2019) |
| بيانات النشر: | BMC |
| سنة النشر: | 2019 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Diabetes mellitus type 1, Islets of Langerhans transplantation, Mesenchymal stem cells, Co-culture, Cytokines, Heme oxygenase 1, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Background Islets of Langerhans transplantation is a promising therapy for type 1 diabetes mellitus, but this technique is compromised by transplantation stresses including inflammation. In other tissues, co-transplantation with mesenchymal stem cells has been shown to reduce damage by improving anti-inflammatory and anti-oxidant defences. Therefore, we probed the protection afforded by bone marrow mesenchymal stem cells to islets under pro-inflammatory cytokine stress. Methods In order to evaluate the cytoprotective potential of mesenchymal stem cells on rat islets, co-cultures were exposed to the interleukin-1, tumour necrosis factor α and interferon γ cocktail for 24 h. Islet viability and functionality tests were performed. Reactive oxygen species and malondialdehyde were measured. Expression of stress-inducible genes acting as anti-oxidants and detoxifiers, such as superoxide dismutases 1 and 2, NAD(P)H quinone oxidoreductase 1, heme oxygenase-1 and ferritin H, was compared to non-stressed cells, and the corresponding proteins were measured. Data were analysed by a two-way ANOVA followed by a Holm-Sidak post hoc analysis. Results Exposure of rat islets to cytokines induces a reduction in islet viability and functionality concomitant with an oxidative status shift with an increase of cytosolic ROS production. Mesenchymal stem cells did not significantly increase rat islet viability under exposure to cytokines but protected islets from the loss of insulin secretion. A drastic reduction of the antioxidant factors heme oxygenase-1 and ferritin H protein levels was observed in islets exposed to the cytokine cocktail with a prevention of this effect by the presence of mesenchymal stem cells. Conclusions Our data evidenced that MSCs are able to preserve islet insulin secretion through a modulation of the oxidative imbalance mediated by heme and iron via heme oxygenase-1 and ferritin in a context of cytokine exposure. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1757-6512 |
| العلاقة: | http://link.springer.com/article/10.1186/s13287-019-1190-4Test; https://doaj.org/toc/1757-6512Test; https://doaj.org/article/6c7c89b474e04d04860bd2723576abe0Test |
| DOI: | 10.1186/s13287-019-1190-4 |
| الإتاحة: | https://doi.org/10.1186/s13287-019-1190-4Test https://doaj.org/article/6c7c89b474e04d04860bd2723576abe0Test |
| رقم الانضمام: | edsbas.C9BDAAE0 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17576512 |
|---|---|
| DOI: | 10.1186/s13287-019-1190-4 |