Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
| العنوان: | Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells |
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| المؤلفون: | Elaine L, Ferguson, Sameza, Naseer, Lydia C, Powell, Joseph, Hardwicke, Fraser I, Young, Bangfu, Zhu, Qian, Liu, Bing, Song, David W, Thomas |
| المصدر: | Stem Cell Research |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | DMF, N,N-dimethyl formamide, Cell Culture Techniques, PLGA, poly(lactic-co-glycolic acid), DMSO, dimethyl sulfoxide, Biodegradable polymers, Article, Olig2, oligodendrocyte transcription factor, MAP2, microtubule-associated protein 2, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, SCI, spinal cord injury, DNA, deoxyribonucleic acid, FPLC, fast protein liquid chromatography, Neural Stem Cells, PBS, phosphate buffered saline, Dextrins, TUNEL, terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labelling, Humans, DMAP, 4-dimethylaminopyridine, Controlled release, EDC, 1-ethyl-3-(3-dimethylaminopropyl carbodiimide hydrochloride), mNSC, mouse neural stem cell, Cell Proliferation, EMEM, eagle's minimum essential media, EGF, epidermal growth factor, bFGF, basic fibroblast growth factor (FGF-2), GFAP, glial fibrillary acidic protein, Cell Differentiation, CLSM, confocal laser scanning microscopy, GPC, gel permeation chromatography, BCA, bicinchoninic acid, HAMC, hyaluronan and methyl cellulose, Fibroblast Growth Factor 2, sulfo-NHS, N-hydroxysulfosuccinimide, FCS, fetal calf serum, GF, growth factor, NSC, neural stem cell, Polymer therapeutics, Growth factors, Hep2, human epidermoid carcinoma |
| الوصف: | An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (~74,000 g/mol; 27.8 mol% succinoylation) conjugated to epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF; or FGF-2) (3.9 and 6.7% w/w protein loading, respectively) to support the expansion and differentiation of stem cells in vitro via sustained, controllable growth factor release. Supplementation of mouse neural stem cells (mNSCs) with dextrin-growth factor conjugates led to greater and prolonged proliferation compared to unbound EGF/bFGF controls, with no detectable apoptosis after 7 days of treatment. Immunocytochemical detection of neural precursor (nestin) and differentiation (Olig2, MAP2, GFAP) markers verified that controlled release of dextrin-conjugated growth factors preserves stem cell properties of mNSCs for up to 7 days. These results show the potential of dextrin-growth factor conjugates for localized delivery of bioactive therapeutic agents to support stem cell expansion and differentiation, and as an adjunct to direct neuronal repair. Graphical abstract Unlabelled Image Highlights • Biodegradable dextrin was conjugated to EGF and bFGF to improve stability. • Sustained release of growth factors potentiated neural stem cell proliferation in vitro. • Dextrin conjugates maintained stem cells in an undifferentiated state. |
| تدمد: | 1876-7753 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::814be96b8e015a323c90b0cbb0a6f467Test https://pubmed.ncbi.nlm.nih.gov/30321831Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid..........814be96b8e015a323c90b0cbb0a6f467 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18767753 |
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