التفاصيل البيبلوغرافية
| العنوان: |
Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines. |
| المؤلفون: |
Zhong, Yixiang, Choi, Taewoong, Kim, Minjae, Jung, Kyoung Hwa, Chai, Young Gyu, Binas, Bert |
| المصدر: |
Stem Cell Research; Jul2018, Vol. 30, p100-112, 13p |
| مستخلص: |
Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells. [ABSTRACT FROM AUTHOR] |
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