TFIID Enables RNA Polymerase II Promoter-Proximal Pausing
| العنوان: | TFIID Enables RNA Polymerase II Promoter-Proximal Pausing |
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| المؤلفون: | Jonathan D. Rubin, Jenna K. Rimel, Charli B. Fant, Zachary L. Maas, Dylan J. Taatjes, Meagan N. Esbin, Michael T. Marr, John Moir, Cecilia B. Levandowski, Kapil Gupta, Andrew Sawyer, Olivia Luyties, Robin D. Dowell, Imre Berger |
| المصدر: | Fant, C B, Levandowski, C B, Gupta, K, Maas, Z L, Moir, J, Rubin, J D, Sawyer, A, Esbin, M N, Rimel, J K, Luyties, O, Marr, M T, Berger, I, Dowell, R D & Taatjes, D J 2020, ' TFIID Enables RNA Polymerase II Promoter-Proximal Pausing ', Molecular Cell, vol. 78, no. 4, pp. 785-793.e8 . https://doi.org/10.1016/j.molcel.2020.03.008Test Mol Cell |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Transcription, Genetic, genetic processes, DSIF, RNA polymerase II, NELF, Article, P-TEFb, 03 medical and health sciences, 0302 clinical medicine, PRO-seq, Animals, Drosophila Proteins, Humans, TAF1, Promoter Regions, Genetic, TRIM-Away, TBP, Molecular Biology, 030304 developmental biology, 0303 health sciences, biology, Chemistry, TFIID, Cell Biology, HCT116 Cells, Cell biology, pausing, Transcription factor II H, biology.protein, Drosophila, Transcription Factor TFIID, Transcription factor II F, RNA Polymerase II, Transcription factor II E, Transcription factor II D, Transcription factor II B, 030217 neurology & neurosurgery, Transcription factor II A, Protein Binding |
| الوصف: | Summary RNA polymerase II (RNAPII) transcription is governed by the pre-initiation complex (PIC), which contains TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, RNAPII, and Mediator. After initiation, RNAPII enzymes pause after transcribing less than 100 bases; precisely how RNAPII pausing is enforced and regulated remains unclear. To address specific mechanistic questions, we reconstituted human RNAPII promoter-proximal pausing in vitro, entirely with purified factors (no extracts). As expected, NELF and DSIF increased pausing, and P-TEFb promoted pause release. Unexpectedly, the PIC alone was sufficient to reconstitute pausing, suggesting RNAPII pausing is an inherent PIC function. In agreement, pausing was lost upon replacement of the TFIID complex with TATA-binding protein (TBP), and PRO-seq experiments revealed widespread disruption of RNAPII pausing upon acute depletion (t = 60 min) of TFIID subunits in human or Drosophila cells. These results establish a TFIID requirement for RNAPII pausing and suggest pause regulatory factors may function directly or indirectly through TFIID. |
| وصف الملف: | application/pdf |
| تدمد: | 1556-5068 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14f69ca34ab9220900fbd1069a208f44Test https://doi.org/10.2139/ssrn.3429345Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....14f69ca34ab9220900fbd1069a208f44 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15565068 |
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