مورد إلكتروني
Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
| العنوان: | Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers |
|---|---|
| المصدر: | Biological Research v.37 n.4 2004 |
| بيانات النشر: | Sociedad de Biología de Chile 2004-01-01 |
| تفاصيل مُضافة: | SCHNEIDER,MARTIN F RODNEY,GEORGE G |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Local discrete elevations in myoplasmic Ca2+ (Ca2+ sparks) arise from the opening of a small group of RyRs. Summation of a large number of Ca2+ sparks gives rise to the whole cell Ca2+ transient necessary for muscle contraction. Unlike sarcoplasmic reticulum vesicle preparations and isolated single channels in artificial membranes, the study of Ca2+ sparks provides a means to understand the regulation of a small group of RyRs in the environment of a functionally intact triad and in the presence of endogenous regulatory proteins. To gain insight into the mechanisms that regulate the gating of RyRs we have utilized laser scanning confocal microscopy to measure Ca2+ sparks in permeabilized frog skeletal muscle fibers. This review summarizes our recent studies using both exogenous (ImperatoxinA and domain peptides) and endogenous (calmodulin) modulators of RyR to gain insight into the number of RyR Ca2+ release channels underlying a Ca2+ spark, how domain-domain interactions within RyR regulate the functional state of the channel as well as gating mechanisms of RyR in living muscle fibers |
| مصطلحات الفهرس: | Excitation-Contraction Coupling, Ca2+ Sparks, IperatoxinA, DP4, Calmodulin |
| URL: | 10.4067/S0716-97602004000400016 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | text/html Sociedad de Biología de Chile English |
| أرقام أخرى: | CN@ oai:scielo:S0716-97602004000400016 1043361258 |
| المصدر المساهم: | CONICYT From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1043361258 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |