دورية أكاديمية
Enhancing scaffold-free spheroid models: 3D cell bioprinting method for metastatic HSC3–Oral squamous carcinoma cell line
| العنوان: | Enhancing scaffold-free spheroid models: 3D cell bioprinting method for metastatic HSC3–Oral squamous carcinoma cell line |
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| المؤلفون: | Taís Bacelar Sacramento de Araújo, Raphael Luís Rocha Nogueira, Leonardo de Oliveira Siquara da Rocha, Iasmin Nogueira Bastos, Rosane Borges Dias, Bruno Solano De Freitas Souza, Daniel William Lambert, Ricardo D. Coletta, Viviane Aline Oliveira Silva, Clarissa A. Gurgel Rocha |
| المصدر: | SLAS Discovery, Vol 29, Iss 4, Pp 100158- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine (General) LCC:Biotechnology |
| مصطلحات موضوعية: | Oral cancer, Spheroids, 3D cell culture, Protocols, Magnetic bioprinting, Medicine (General), R5-920, Biotechnology, TP248.13-248.65 |
| الوصف: | 3D in vitro systems offer advantages over the shortcomings of two-dimensional models by simulating the morphological and functional features of in vivo-like environments, such as cell-cell and cell-extracellular matrix interactions, as well as the co-culture of different cell types. Nevertheless, these systems present technical challenges that limit their potential in cancer research requiring cell line- and culture-dependent standardization. This protocol details the use of a magnetic 3D bioprinting method and other associated techniques (cytotoxicity assay and histological analysis) using oral squamous cell carcinoma cell line, HSC3, which offer advantages compared to existing widely used approaches. This protocol is particularly timely, as it validates magnetic bioprinting as a method for the rapid deployment of 3D cultures as a tool for compound screening and development of heterotypic cultures such as co-culture of oral squamous cell carcinoma cells with cancer-associated fibroblasts (HSC3/CAFs). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2472-5552 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2472555224000200Test; https://doaj.org/toc/2472-5552Test |
| DOI: | 10.1016/j.slasd.2024.100158 |
| الوصول الحر: | https://doaj.org/article/d85631babf104765a0217f2302f5a181Test |
| رقم الانضمام: | edsdoj.85631babf104765a0217f2302f5a181 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24725552 |
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| DOI: | 10.1016/j.slasd.2024.100158 |