Evaluation of a Product Containing Xyloglucan and Pea Protein on Skin Barrier Permeability
| العنوان: | Evaluation of a Product Containing Xyloglucan and Pea Protein on Skin Barrier Permeability |
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| المؤلفون: | Marika Lanza, Michela Campolo, Emanuela Esposito, Salvatore Cuzzocrea, Alessio Ardizzone, Sarah Adriana Scuderi, Giovanna Casili, Alessia Filippone, Irene Paterniti |
| المصدر: | Skin Pharmacology and Physiology. 33:231-236 |
| بيانات النشر: | S. Karger AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Keratinocytes, Staphylococcus aureus, Membrane permeability, Physiology, Dermatology, medicine.disease_cause, Bacterial Adhesion, Permeability, Microbiology, chemistry.chemical_compound, Multiplicity of infection, Lactate dehydrogenase, medicine, HaCaT Cells, Humans, Glucans, Skin, Pharmacology, biology, Pea protein, Skin barrier, Xyloglucan, General Medicine, biology.organism_classification, HaCaT, chemistry, Staphylococcal Skin Infections, Xylans, Bacteria, Pea Proteins |
| الوصف: | Objective: The skin acts as a mechanical and protective barrier against viral, fungal, and bacterial infections. Skin conditions such as atopic dermatitis and psoriasis are characterized by alterations of the skin barrier, often caused by injury and by bacterial infections. In the last years, non-pharmacological interventions have gained great importance in epidermis-related diseases. Xyloglucan (XG) is a polysaccharide that possesses a “mucin-like” molecular structure that confers mucoadhesive properties, allowing XG-containing formulations to act as a protective barrier for the management of different diseases. Moreover, there is also increasing interest in the use of proteins due to their film-forming features. This study aimed to evaluate the barrier-protective properties of a product containing XG and pea protein (PP) in an in vitro model, assessing its effects on the membrane permeability of keratinocytes infected by Staphylococcus aureus. Methods: HaCaT keratinocytes were pretreated with XG and PP for 3 h and then infected with S. aureus cells (106 bacteria/well) at a multiplicity of infection of 10 for 1 h. The number of bacterial colonies and membrane integrity were measured, respectively. Results: We observed that pretreatment with XG and PP in human HaCaT keratinocytes infected with S. aureus significantly increased trans-epithelial electrical resistance (a marker of skin barrier function) measurement, reduced lucifer yellow (a marker of membrane integrity) permeation across the monolayer, and released lactate dehydrogenase (a marker of tissue damage). Moreover, XG and PP pretreatment was able to reduce bacterial adherence, avoiding S. aureus infection. Conclusion: In summary, we demonstrated that the product containing XG and PP was able to maintain barrier permeability preserving its integrity, and therefore, it can be considered as an interesting approach for the management of epidermis-related diseases. |
| تدمد: | 1660-5535 1660-5527 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d01c4918ef39334b11dfb7f8ba83588Test https://doi.org/10.1159/000509372Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d01c4918ef39334b11dfb7f8ba83588 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16605535 16605527 |
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