دورية أكاديمية
Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)
| العنوان: | Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas) |
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| المؤلفون: | KOU Xiuying, ZHOU Baoqing, CHEN Ling, DAI Jingsha, ZHANG Jumei, WU Qingping |
| المصدر: | Shipin Kexue, Vol 44, Iss 7, Pp 313-323 (2023) |
| بيانات النشر: | China Food Publishing Company, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Food processing and manufacture |
| مصطلحات موضوعية: | clustered regularly interspaced short palindromic repeats (crispr)/crispr-associated protein, foodborne pathogens, trans-cleavage, rapid detection technology, Food processing and manufacture, TP368-456 |
| الوصف: | The early screening and rapid detection of foodborne pathogens is critical to food safety and public health. However, the traditional detection method is cumbersome, time-consuming and laborious, and cannot meet the requirement of rapid detection. CRISPR/Cas, composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas), is an immune system widely present in bacteria and archaea, which provides a novel pathway for low-cost, rapid, specific and sensitive detection of foodborne pathogens due to its efficient and specific sequence recognition and cleavage activity. This review article introduces the principle, mechanism and development of the CRISPR/Cas system, and summarizes the latest progresses in the rapid detection of foodborne pathogens based on the CRISPR/Cas system combined with different result reporting methods in recent years. Its advantages, limitations and future development prospects are also discussed. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Chinese |
| تدمد: | 1002-6630 |
| العلاقة: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-7-036.pdfTest; https://doaj.org/toc/1002-6630Test |
| DOI: | 10.7506/spkx1002-6630-20220316-195 |
| الوصول الحر: | https://doaj.org/article/06e1e093fc0e417d97f2e2cb05d11600Test |
| رقم الانضمام: | edsdoj.06e1e093fc0e417d97f2e2cb05d11600 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 10026630 |
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| DOI: | 10.7506/spkx1002-6630-20220316-195 |