التفاصيل البيبلوغرافية
| العنوان: |
Optical Biosensor for the Detection of Infectious Diseases Using the Copolymer F8T2 with Application to COVID-19. |
| المؤلفون: |
Bassi, Maiara de Jesus1 (AUTHOR) mai.bassi89@gmail.com, Araujo Todo Bom, Maritza2 (AUTHOR) maritzabom@gmail.com, Terribile Budel, Maria Luisa2 (AUTHOR) malu.budel@ufpr.br, Maltempi de Souza, Emanuel2 (AUTHOR) souzaem@ufpr.br, Müller dos Santos, Marcelo2 (AUTHOR) marcelomuller@ufpr.br, Roman, Lucimara Stolz1 (AUTHOR) |
| المصدر: |
Sensors (14248220). Aug2022, Vol. 22 Issue 15, p5673-N.PAG. 10p. |
| مصطلحات موضوعية: |
*COVID-19, *SARS-CoV-2, *COMMUNICABLE diseases, *BIOSENSORS |
| مستخلص: |
The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has accelerated the development of biosensors based on new materials and techniques. Here, we present our effort to develop a fast and affordable optical biosensor using photoluminescence spectroscopy for anti-SARS-CoV-2 antibody detection. The biosensor was fabricated with a thin layer of the semiconductor polymer Poly[(9,9-di-n-octylfluorenyl-2,7-diyl)-alt-2,2′-bithiophene-5,5′-diyl)] (F8T2) as a signal transducer material. We mounted the biosensors by depositing a layer of F8T2 and an engineered version of RBD from the SARS-CoV-2 spike protein with a tag to promote hydrophobic interaction between the protein and the polymeric surface. We validated the biosensor sensitivity with decreasing anti-RBD polyclonal IgG concentrations and challenged the biosensor specificity with human serum samples from both COVID-19 negative and positive individuals. The antibody binding to the immobilized antigen shifted the F8T2 photoluminescence spectrum even at the low concentration of 0.0125 µg/mL. A volume as small as one drop of serum (100 µL) was sufficient to distinguish a positive from a negative sample without requiring multiple washing steps and secondary antibody reactions. [ABSTRACT FROM AUTHOR] |
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