دورية أكاديمية

Living Matter Observations with a Novel Hyperspectral Supercontinuum Confocal Microscope for VIS to Near-IR Reflectance Spectroscopy

التفاصيل البيبلوغرافية
العنوان: Living Matter Observations with a Novel Hyperspectral Supercontinuum Confocal Microscope for VIS to Near-IR Reflectance Spectroscopy
المؤلفون: Francesca R. Bertani, Luisa Ferrari, Valentina Mussi, Elisabetta Botti, Antonio Costanzo, Stefano Selci
المصدر: Sensors, Vol 13, Iss 11, Pp 14523-14542 (2013)
بيانات النشر: MDPI AG, 2013.
سنة النشر: 2013
المجموعة: LCC:Chemical technology
مصطلحات موضوعية: confocal, hyperspectral imaging, spectroscopy, Chemical technology, TP1-1185
الوصف: A broad range hyper-spectroscopic microscope fed by a supercontinuum laser source and equipped with an almost achromatic optical layout is illustrated with detailed explanations of the design, implementation and data. The real novelty of this instrument, a confocal spectroscopic microscope capable of recording high resolution reflectance data in the VIS-IR spectral range from about 500 nm to 2.5 μm wavelengths, is the possibility of acquiring spectral data at every physical point as defined by lateral coordinates, X and Y, as well as at a depth coordinate, Z, as obtained by the confocal optical sectioning advantage. With this apparatus we collect each single scanning point as a whole spectrum by combining two linear spectral detector arrays, one CCD for the visible range, and one InGaAs infrared array, simultaneously available at the sensor output channel of the home made instrument. This microscope has been developed for biomedical analysis of human skin and other similar applications. Results are shown illustrating the technical performances of the instrument and the capability in extracting information about the composition and the structure of different parts or compartments in biological samples as well as in solid statematter. A complete spectroscopic fingerprinting of samples at microscopic level is shown possible by using statistical analysis on raw data or analytical reflectance models based on Abelés matrix transfer methods.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1424-8220
العلاقة: http://www.mdpi.com/1424-8220/13/11/14523Test; https://doaj.org/toc/1424-8220Test
DOI: 10.3390/s131114523
الوصول الحر: https://doaj.org/article/0de6eb3bff3e498f93c12bfdb0920655Test
رقم الانضمام: edsdoj.0de6eb3bff3e498f93c12bfdb0920655
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14248220
DOI:10.3390/s131114523