دورية أكاديمية
Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis
| العنوان: | Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis |
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| المؤلفون: | Hartmann, Rico M., Schaepe, Sieke, Nübel, Daniel, Petersen, Arne C., Bertolini, Martina, Vasilev, Jana, Küster, Helge, Hohnjec, Natalija |
| المصدر: | Scientific Reports 9 (2019) |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2019 |
| المجموعة: | Institutional Repository of Leibniz Universität Hannover |
| مصطلحات موضوعية: | Arbuscular mycorrhiza, Fluorescence imaging, Gene expression profiling, Plant molecular biology, Plant signalling, ddc:570 |
| الوصف: | To improve access to limiting nutrients, the vast majority of land plants forms arbuscular mycorrhizal (AM) symbioses with Glomeromycota fungi. We show here that AM-related GRAS transcription factors from different subgroups are upregulated during a time course of mycorrhization. Based on expression studies in mutants defective in arbuscule branching (ram1-1, with a deleted MtRam1 GRAS transcription factor gene) or in the formation of functional arbuscules (pt4-2, mutated in the phosphate transporter gene MtPt4), we demonstrate that the five AM-related GRAS transcription factor genes MtGras1, MtGras4, MtGras6, MtGras7, and MtRad1 can be differentiated by their dependency on MtRAM1 and MtPT4, indicating that the network of AM-related GRAS transcription factors consists of at least two regulatory modules. One module involves the MtRAM1- and MtPT4-independent transcription factor MtGRAS4 that activates MtGras7. Another module is controlled by the MtRAM1- and MtPT4-dependent transcription factor MtGRAS1. Genome-wide expression profiles of mycorrhized MtGras1 knockdown and ram1-1 roots differ substantially, indicating different targets. Although an MtGras1 knockdown reduces transcription of AM-related GRAS transcription factor genes including MtRam1 and MtGras7, MtGras1 overexpression alone is not sufficient to activate MtGras genes. MtGras1 knockdown roots display normal fungal colonization, with a trend towards the formation of smaller arbuscules. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | ESSN:2045-2322; http://dx.doi.org/10.15488/4843Test; https://www.repo.uni-hannover.de/handle/123456789/4886Test |
| DOI: | 10.15488/4843 |
| الإتاحة: | https://doi.org/10.15488/4843Test https://doi.org/10.1038/s41598-019-40214-4Test https://www.repo.uni-hannover.de/handle/123456789/4886Test |
| حقوق: | CC BY 4.0 Unported ; https://creativecommons.org/licenses/by/4.0Test/ ; frei zugänglich |
| رقم الانضمام: | edsbas.ADE532E3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.15488/4843 |
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