Improving orthotopic mouse models of patient-derived breast cancer brain metastases by a modified intracarotid injection method
| العنوان: | Improving orthotopic mouse models of patient-derived breast cancer brain metastases by a modified intracarotid injection method |
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| المؤلفون: | Zongming Liu, Sheheryar Kabraji, Zhenning Liu, Shaozhen Xie, Peichen Pan, Yanzhi Wang, Jing Ni, Jean J. Zhao |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, medicine.medical_specialty, lcsh:Medicine, Translational research, Tumor cells, Breast Neoplasms, Kaplan-Meier Estimate, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Breast cancer, Internal medicine, Exome Sequencing, medicine, Biomarkers, Tumor, Tumor Microenvironment, Animals, Humans, lcsh:Science, Multidisciplinary, Extramural, business.industry, Brain Neoplasms, lcsh:R, medicine.disease, Immunohistochemistry, 3. Good health, Disease Models, Animal, 030104 developmental biology, Blood-Brain Barrier, lcsh:Q, Female, business, 030217 neurology & neurosurgery, Brain metastasis |
| الوصف: | Breast cancer brain metastasis (BCBM) remains a major clinical problem. Approximately 10–16% of patients with breast cancer develop brain metastases (BCBM). However, no systemic therapy has gained regulatory approval for the specific treatment of BCBM and this remains an area of persistent, unmet medical need. Rapid, predictive and clinically-relevant animal models are critical to study the biology of brain metastases and to identify effective therapeutic approaches for patients with BCBM. Here, we describe a method for efficient establishment of orthotopic mouse models of patient-derived brain metastases via an improved intracarotid injection protocol that permits tumor cell growth in the unique brain microenvironment without compromising the blood-brain barrier (BBB). We demonstrate that our newly improved models of patient-derived brain metastases recapitulate the histologic, molecular, and genetic characteristics of their matched patient tumor specimens and thus represent a potentially powerful tool for pre-clinical and translational research. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0ed8d38ff39f1e9e1837dbcb7e83dbaTest https://pubmed.ncbi.nlm.nih.gov/30679540Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d0ed8d38ff39f1e9e1837dbcb7e83dba |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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