Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
| العنوان: | Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes |
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| المؤلفون: | Anna-Karin Larsson-Callerfelt, Irene Rodríguez-Meizoso, Oskar Rosmark, Alicia Gil-Ramírez, Gunilla Westergren-Thorsson, Karl Swärd, Peter Spégel |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Quality of life, 0301 basic medicine, Swine, lcsh:Medicine, 02 engineering and technology, Pulmonary Artery, Article, Extracellular matrix, 03 medical and health sciences, chemistry.chemical_compound, Medical research, In vivo, Animals, Solvent composition, lcsh:Science, Analytical biochemistry, Multidisciplinary, Decellularization, Chromatography, Tissue Scaffolds, lcsh:R, Carbon Dioxide, 021001 nanoscience & nanotechnology, Lipids, Supercritical fluid, Extracellular Matrix, 3. Good health, 030104 developmental biology, chemistry, Carbon dioxide, lcsh:Q, 0210 nano-technology |
| الوصف: | Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO2-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO2-EtOH-H2O fluids (average molar composition, ΧCO2 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO2-limonene fluids (ΧCO2 0.88) and neat supercritical CO2 (scCO2) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO2-limonene followed by enzyme treatment, resulted in efficient DNA removal. The structure of elastic fibers was preserved after pressurized treatment, regardless solvent composition. In conclusion, pressurized CO2-ethanol fluids offer an efficient tool for delipidation in bio-scaffold production, while pressurized CO2-limonene fluids facilitate subsequent enzymatic removal of DNA. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9a0b09dcbe35dced9957d99408b216fTest http://link.springer.com/article/10.1038/s41598-020-60827-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b9a0b09dcbe35dced9957d99408b216f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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