التفاصيل البيبلوغرافية
| العنوان: |
Artesunate acts through cytochrome c to inhibit growth of pediatric AML cells. |
| المؤلفون: |
Hill, Kristen S., Schuler, Erin E., Ellingson, Sally R., Kolesar, Jill M. |
| المصدر: |
Scientific Reports; 12/16/2023, Vol. 13 Issue 1, p1-13, 13p |
| مصطلحات موضوعية: |
CYTOCHROME c, ACUTE myeloid leukemia, MITOCHONDRIAL proteins, ARTEMISININ derivatives, CHINESE medicine |
| مستخلص: |
Artesunate is a derivative of artemisinin, an active compound isolated from Artemisia annua which has been used in Traditional Chinese Medicine and to treat malaria worldwide. Artemisinin derivatives have exhibited anti-cancer activity against both solid tumors and leukemia. The direct target(s) of artesunate are controversial; although, heme-bound proteins in the mitochondria have been implicated. We utilized computational modeling to calculate the predicted binding score of artesunate with heme-bound mitochondrial proteins and identified cytochrome c as potential artesunate target. UV–visible spectroscopy showed changes in the absorbance spectrum, and thus protein structure, when cytochrome c was incubated with artesunate. Artesunate induces apoptosis, disrupts mitochondrial membrane potential, and is antagonized by methazolamide in pediatric AML cells indicating a probable mechanism of action involving cytochrome c. We utilized a multi-disciplinary approach to show that artesunate can interact with and is dependent on cytochrome c release to induce cell death in pediatric AML cell lines. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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