دورية أكاديمية

Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

التفاصيل البيبلوغرافية
العنوان: Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
المؤلفون: Elena López-Ruiz, Seshasailam Venkateswaran, Macarena Perán, Gema Jiménez, Salvatore Pernagallo, Juan J. Díaz-Mochón, Olga Tura-Ceide, Francisco Arrebola, Juan Melchor, Juan Soto, Guillermo Rus, Pedro J. Real, María Diaz-Ricart, Antonio Conde-González, Mark Bradley, Juan A. Marchal
المصدر: Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
بيانات النشر: Nature Portfolio
سنة النشر: 2017
المجموعة: Directory of Open Access Journals: DOAJ Articles
مصطلحات موضوعية: Medicine, Science
الوصف: Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 2045-2322
العلاقة: https://doi.org/10.1038/s41598-017-00294-6Test; https://doaj.org/toc/2045-2322Test; https://doaj.org/article/277e4c5adca947e99621d87e4cd3c23aTest
DOI: 10.1038/s41598-017-00294-6
الإتاحة: https://doi.org/10.1038/s41598-017-00294-6Test
https://doaj.org/article/277e4c5adca947e99621d87e4cd3c23aTest
رقم الانضمام: edsbas.2B52011C
قاعدة البيانات: BASE
الوصف
تدمد:20452322
DOI:10.1038/s41598-017-00294-6