دورية أكاديمية
Nanopore-based detection and characterization of yam viruses
| العنوان: | Nanopore-based detection and characterization of yam viruses |
|---|---|
| المؤلفون: | Filloux, Denis, Fernandez, Emmanuel, Loire, Etienne, Claude, Lisa, Galzi, Serge, Candresse, Thierry, Winter, Stephan, Jeeva, M.L., Makeshkumar, T., Martin, Darren Patrick, Roumagnac, Philippe |
| المصدر: | Scientific Reports |
| سنة النشر: | 2018 |
| المجموعة: | CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) |
| مصطلحات موضوعية: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, patate douce, virus, séquence nucléotidique, http://aims.fao.org/aos/agrovoc/c_14729Test, http://aims.fao.org/aos/agrovoc/c_8262Test, http://aims.fao.org/aos/agrovoc/c_27583Test |
| الوصف: | We here assessed the capability of the MinION sequencing approach to detect and characterize viruses infecting a water yam plant. This sequencing platform consistently revealed the presence of several plant virus species, including Dioscorea bacilliform virus, Yam mild mosaic virus and Yam chlorotic necrosis virus. A potentially novel ampelovirus was also detected by a complimentary Illumina sequencing approach. The full-length genome sequence of yam chlorotic necrosis virus was determined using Sanger sequencing, which enabled determination of the coverage and sequencing accuracy of the MinION technology. Whereas the total mean sequencing error rate of yam chlorotic necrosis virus-related MinION reads was 11.25%, we show that the consensus sequence obtained either by de novo assembly or after mapping the MinION reads on the virus genomic sequence was >99.8% identical with the Sanger-derived reference sequence. From the perspective of potential plant disease diagnostic applications of MinION sequencing, these degrees of sequencing accuracy demonstrate that the MinION approach can be used to both reliably detect and accurately sequence nearly full-length positive-sense single-strand polyadenylated RNA plant virus genomes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | http://agritrop.cirad.fr/590016Test/; Nanopore-based detection and characterization of yam viruses. Filloux Denis, Fernandez Emmanuel, Loire Etienne, Claude Lisa, Galzi Serge, Candresse Thierry, Winter Stephan, Jeeva M.L., Makeshkumar T., Martin Darren Patrick, Roumagnac Philippe. 2018. Scientific Reports, 8:17879, 11 p.https://doi.org/10.1038/s41598-018-36042-7Test; http://agritrop.cirad.fr/590016/1/Filloux_Scientific_Reports_Nanopore_2018.pdfTest |
| DOI: | 10.1038/s41598-018-36042-7 |
| الإتاحة: | https://doi.org/10.1038/s41598-018-36042-7Test http://agritrop.cirad.fr/590016Test/ http://agritrop.cirad.fr/590016/1/Filloux_Scientific_Reports_Nanopore_2018.pdfTest |
| حقوق: | cc_by ; info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.FE2B9EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-018-36042-7 |
|---|