Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence
| العنوان: | Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence |
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| المؤلفون: | Saleh A. Eifan, Ibrahim O. Alanazi, Atif Hanif, Abdulkarim Alhetheel, Ibrahim Al-Ashkar, Islam Nour |
| المصدر: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| بيانات النشر: | Nature Publishing Group UK, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Rotavirus, Thermotolerance, Veterinary medicine, Genotype, Molecular biology, Science, 030106 microbiology, Biology, Irrigation water, Microbiology, Article, Rotavirus Infections, 03 medical and health sciences, Microbial ecology, Virology, Human rotavirus, medicine, Humans, Amino Acid Sequence, Antigens, Viral, Phylogeny, Multidisciplinary, Phylogenetic tree, Environmental microbiology, Genetic Variation, Seasonality, medicine.disease, Fecal coliform, 030104 developmental biology, Wastewater, DNA, Viral, Medicine, Capsid Proteins |
| الوصف: | The routine evaluation of water environments is necessary to manage enteric virus-mediated fecal contamination and the possible emergence of novel variants. Here, we detected human rotavirus A (HRVA) circulating in two wastewater treatment plants, two lakes, irrigation water and a wastewater landfill located in Riyadh. VP7-derived surface protein sequences were assessed by phylogenetic analyses and inspection of thermotolerance-mediated secondary structure and seasonal variation. HRVA was most prevalent at An-Nazim wastewater landfill (AN-WWLF; 63.89%). Phylogenetic analyzes revealed the predominance of HRVA G2 lineage for the first time in Saudi Arabia. Moreover, a single HRVA sequence (2B64I-ANLF3/2018) was recovered at 45 °C from AN-WWLF; secondary structure prediction indicated that this sequence was thermotolerant with a high hydrophobicity, an absence of Ramachandran outliers, and a higher content of proline patches on the protein surface. Varied relationships were significantly observed between sampling areas influenced by temperature ranges (p p = 0.01). Our study extends the knowledge of currently circulating HRVA genotypes, and indicates the probable emergence of thermotolerant strains and seasonally mediated HRVA prevalence. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b899570a480d8d7823dc1ceff9c58236Test http://europepmc.org/articles/PMC8190275Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b899570a480d8d7823dc1ceff9c58236 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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