المؤلفون: Agata Rolnik, Anna Stochmal, Bartosz Skalski, Beata Olas

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)

مصطلحات موضوعية: Blood Platelets, Cell biology, Antioxidant, Cell Survival, Science, medicine.medical_treatment, Blood Donors, Biochemistry, Article, Lipid peroxidation, Cucurbita pepo, chemistry.chemical_compound, Platelet Adhesiveness, Cucurbita, Vegetables, medicine, TBARS, Humans, Platelet, Platelet activation, Food science, Whole blood, Hemostasis, Multidisciplinary, biology, L-Lactate Dehydrogenase, Plant Extracts, food and beverages, Thrombosis, biology.organism_classification, Healthy Volunteers, chemistry, Fruit, Seeds, Medicine, Lipid Peroxidation, Cucumis sativus, Platelet Aggregation Inhibitors, Squash, Signal Transduction

الوصف: Increased blood platelet activation plays an important role in cardiovascular diseases (CVDs). Recent experiments indicate that certain fruits and vegetables, including onion, garlic, and beetroot, have anti-platelet potential and therefore may reduce the likelihood of CVDs. While vegetables from the Cucuritaceae family are known to exerting beneficial antioxidant and anti-inflammatory effects, their effects on blood platelet activation are poorly understood. Therefore, the aim of the present study was to determine the effect on platelet adhesion of preparations from selected cucurbits: pumpkin (Cucurbita pepo; fruit without seeds), zucchini (Cucurbita pepo convar. giromontina; fruit with seeds), cucumber (Cucumis sativus; fruit with seeds), white pattypan squash (Cucurbita pepo var. patisoniana; fruit without seeds) and yellow pattypan squash (Cucurbita pepo var. patisoniana, fruit without seeds). It also evaluates the activity of these preparations on enzymatic lipid peroxidation in thrombin-activated washed blood platelets by TBARS assay. The study also determines the anti-platelet properties of these five cucurbit preparations in whole blood by flow cytometry and with the total thrombus-formation analysis system (T-TAS) and evaluates the cytotoxicity of the tested preparations against platelets based on LDH activity. The results indicate that the yellow Cucurbita pepo var. patisoniana preparation demonstrated stronger anti-platelet properties than the other tested preparations, reducing the adhesion of thrombin-activated platelets to collagen/fibrinogen, and inhibiting arachidonic acid metabolism and GPIIb/IIIa expression on 10 µM ADP-activated platelets. None of the preparations was found to cause platelet lysis. Our findings provide new information on the anti-platelet activity of the tested cucurbit preparations and their potential for treating CVDs associated with platelet hyperactivity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d48a7df058cf6fb5b32561d9b93f9c48Test
http://europepmc.org/articles/PMC8608840Test

المؤلفون: Rémy Froissart, Shaun L. Barnabas, Heather B. Jaspan, Linda G. Bekker, Shameem Z. Jaumdally, Hoyam Gamieldien, Glenda Gray, Jo-Ann S. Passmore, Andrea G. Abrahams, Lindi Masson, Monalisa T. Manhanzva, Smritee Dabee

المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports

مصطلحات موضوعية: 0301 basic medicine, Adult, Adolescent, medicine.medical_treatment, 030106 microbiology, lcsh:Medicine, Inflammation, Biology, medicine.disease_cause, Article, Microbiology, Proinflammatory cytokine, 03 medical and health sciences, chemistry.chemical_compound, South Africa, Young Adult, fluids and secretions, Lactate dehydrogenase, Lactobacillus, medicine, Gardnerella vaginalis, Humans, lcsh:Science, Gram-Positive Bacterial Infections, Multidisciplinary, lcsh:R, food and beverages, Vaginosis, Bacterial, Antimicrobial, biology.organism_classification, Bacterial host response, Lactic acid, 030104 developmental biology, Cytokine, chemistry, Vagina, Cytokines, Female, lcsh:Q, medicine.symptom, Bacterial infection

الوصف: Female genital tract (FGT) inflammation increases HIV infection susceptibility. Non-optimal cervicovaginal microbiota, characterized by depletion of Lactobacillus species and increased bacterial diversity, is associated with increased FGT cytokine production. Lactobacillus species may protect against HIV partly by reducing FGT inflammation. We isolated 80 lactobacilli from South African women with non-optimal (Nugent 4–10; n = 18) and optimal microbiota (Nugent 0–3; n = 14). Cytokine production by vaginal epithelial cells in response to lactobacilli in the presence and absence of Gardnerella vaginalis was measured using Luminex. Adhesion to vaginal epithelial cells, pH, D/L-lactate production and lactate dehydrogenase relative abundance were assessed. Lactobacilli from women with non-optimal produced less lactic acid and induced greater inflammatory cytokine production than those from women with optimal microbiota, with IL-6, IL-8, IL-1α, IL-1β and MIP-1α/β production significantly elevated. Overall, lactobacilli suppressed IL-6 (adjusted p G. vaginalis. Cytokine responses to the lactobacilli were inversely associated with lactobacilli adhesion to epithelial cells and D-lactate dehydrogenase relative abundance. Thus, while cervicovaginal lactobacilli reduced the production of the majority of inflammatory cytokines in response to G. vaginalis, isolates from women with non-optimal microbiota were more inflammatory and produced less lactic acid than isolates from women with optimal microbiota.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1406daeda693a00eceecd5b45fcd1ac0Test
http://link.springer.com/article/10.1038/s41598-020-62184-8Test

المؤلفون: Tsung-Meng Wu, Kuan-Chu Chen, Fan-Hua Nan, Yu-Sheng Wu

المصدر: Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Scientific Reports

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_treatment, Interleukin-1beta, Macrophage polarization, lcsh:Medicine, 02 engineering and technology, Galactans, Article, 03 medical and health sciences, chemistry.chemical_compound, Mice, Polysaccharides, Lactate dehydrogenase, medicine, Animals, Secretion, lcsh:Science, RAW 264.7 Cells, Multidisciplinary, Macrophages, lcsh:R, NF-kappa B, Interleukin, 021001 nanoscience & nanotechnology, Molecular biology, 030104 developmental biology, Real-time polymerase chain reaction, Cytokine, chemistry, Rhodophyta, Tumor necrosis factor alpha, lcsh:Q, Biomaterials - cells, 0210 nano-technology

الوصف: In this study, the effects of acetyl-xylogalactan extracted from Sarcodia suieae on RAW 264.7 macrophage polarisation were evaluated. This extracted acetyl-xylogalactan had a monosaccharide composition of 91% galactose and 9% xylose, with polysaccharide and acetyl contents of 80.6% and 19.3%, respectively. MALDI–TOF mass spectrometry and NMR spectroscopy revealed the molecular weight of the acetyl-xylogalactan to be 88.5 kDa. After acetyl-xylogalactan treatment, RAW 264.7 macrophage polarisation was noted, along with enhanced phagocytic ability. Furthermore, the Cell Counting Kit-8 (CCK-8) assay was performed and the results demonstrated non-significant alteration in lactate dehydrogenase levels in the treated cells. Next, interleukin (IL) 1β, TNF, and Malt-1 expression in RAW 264.7 macrophages treated with the S. suieae acetyl-xylogalactan was investigated through real-time quantitative polymerase chain reaction, and the results demonstrated that S. suieae acetyl-xylogalactan induced IL-1β and Malt-1 expression. RNA sequencing analysis results indicated the S. suieae acetyl-xylogalactan positively regulated cytokine production and secretion, protein secretion, and response to IL-1 activation, based on the observed GO terms. The predicted target genes in the GO enrichment analysis were found to upregulate NF-κB signalling and M0 to M1 macrophage conversion through the observed cytokine production. Thus, acetyl-xylogalactan can positively regulate RAW 264.7 macrophage polarisation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e33cd3747de160431887d91ffe509467Test
http://link.springer.com/article/10.1038/s41598-019-56246-9Test

المؤلفون: Farid Zayeri, Anoshirvan Kazemnejad, Abbas Hajifathali, Aliakbar Rasekhi, Farzaneh Tavakoli, Maedeh Amini

المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Scientific Reports

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, medicine.medical_specialty, Allogeneic transplantation, Adolescent, medicine.medical_treatment, Graft vs Host Disease, lcsh:Medicine, Hematopoietic stem cell transplantation, Gastroenterology, Article, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, Internal medicine, medicine, Humans, Transplantation, Homologous, lcsh:Science, Creatinine, Multidisciplinary, L-Lactate Dehydrogenase, Receiver operating characteristic, business.industry, Haematopoietic stem cells, Statistics, lcsh:R, Hematopoietic Stem Cell Transplantation, Diagnostic markers, Gold standard (test), Middle Aged, medicine.disease, Uric Acid, Transplantation, 030104 developmental biology, Graft-versus-host disease, ROC Curve, chemistry, 030220 oncology & carcinogenesis, Female, lcsh:Q, business, Biomarkers

الوصف: Graft-versus-host disease (GVHD) is one of the major causes of morbidity and mortality in 25–70% of patients. The gold standard (GS) test to confirm the diagnosis of GVHD has some limitations. The current study was conducted to evaluate the accuracy of three serum markers in diagnosing GVHD without a GS. 94 patients who were hospitalized for allogeneic transplantation were studied. Mean levels from day of haematopoietic stem cell transplantation (HSCT) to discharge of serum uric acid (UA), lactate dehydrogenase (LDH), and creatinine (Cr) were measured for all participants. We adapted a Bayesian latent class analysis to modelling the results of each marker and combination of markers. The Sensitivity, Specificity, and area under receiver operating characteristic curve (AUC) for LDH were as 51%, 81%, and 0.70, respectively. For UA, the Sensitivity, Specificity, and AUC were 54%, 75%, and 0.71, respectively. The estimated Sensitivity, Specificity, and AUC of Cr were 72%, 94%, and 0.86, respectively. Adjusting for covariates, the combined Sensitivity, Specificity, and AUC of the optimal marker combination were 76%, 83%, and 0.94, respectively. To conclude, our findings suggested that Cr had the strongest diagnosis power for GVHD. Moreover, the classification accuracy of the three-marker combination outperforms the other combinations.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d92885841f932bb61f3ec955d333fc0Test
https://doi.org/10.1038/s41598-020-60524-2Test

المؤلفون: Seow Yen Tan, Matthew E. Cove, Yee Sin Leo, David C. Lye, Duu Wen Sewa, Benjamin Choon Heng Ho, Vernon J. Lee, Barnaby Edward Young, Chee Keat Tan, John A Abisheganaden, Po Ying Chia, Li Min Ling, Cher Heng Tan, Shirin Kalimuddin, Louis Y.A. Chai, Tsin W. Yeo, Jiashen Loh, Roshni Sadashiv Gokhale, Raymond T. P. Lin, Jensen Jiansheng Ng, Vui Kian Ho, Ser Hon Puah, Surinder Pada, Purnima Parthasarathy

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)

مصطلحات موضوعية: Adult, Male, ARDS, Respiratory distress syndrome, Neutrophils, Science, medicine.medical_treatment, 030204 cardiovascular system & hematology, Severity of Illness Index, Article, 03 medical and health sciences, Plateau pressure, 0302 clinical medicine, Respiratory Rate, Severity of illness, Medicine, Intubation, Humans, 030212 general & internal medicine, Prospective Studies, Prospective cohort study, Mechanical ventilation, Singapore, Multidisciplinary, L-Lactate Dehydrogenase, business.industry, SARS-CoV-2, COVID-19, Middle Aged, medicine.disease, Respiration, Artificial, Intensive Care Units, C-Reactive Protein, Dyspnea, Logistic Models, ROC Curve, Viral infection, Anesthesia, Area Under Curve, Breathing, Absolute neutrophil count, Female, business

الوصف: We aim to describe a case series of critically and non-critically ill COVID-19 patients in Singapore. This was a multicentered prospective study with clinical and laboratory details. Details for fifty uncomplicated COVID-19 patients and ten who required mechanical ventilation were collected. We compared clinical features between the groups, assessed predictors of intubation, and described ventilatory management in ICU patients. Ventilated patients were significantly older, reported more dyspnea, had elevated C-reactive protein and lactate dehydrogenase. A multivariable logistic regression model identified respiratory rate (aOR 2.83, 95% CI 1.24–6.47) and neutrophil count (aOR 2.39, 95% CI 1.34–4.26) on admission as independent predictors of intubation with area under receiver operating characteristic curve of 0.928 (95% CI 0.828–0.979). Median APACHE II score was 19 (IQR 17–22) and PaO2/FiO2 ratio before intubation was 104 (IQR 89–129). Median peak FiO2 was 0.75 (IQR 0.6–1.0), positive end-expiratory pressure 12 (IQR 10–14) and plateau pressure 22 (IQR 18–26) in the first 24 h of ventilation. Median duration of ventilation was 6.5 days (IQR 5.5–13). There were no fatalities. Most COVID-19 patients in Singapore who required mechanical ventilation because of ARDS were extubated with no mortality.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db27aa18e982d36dc6b944c09ccea904Test
http://europepmc.org/articles/PMC8021583Test

المؤلفون: Andrew V. Schally, Annalisa Chiavaroli, Riccarda Granata, Sheila Leone, Claudio Ferrante, Wei Sha, Serena Veschi, Giustino Orlando, Renzhi Cai, Valentina Di Valerio, Iacopo Gesmundo, Lucia Recinella, Rossano Lattanzio, Luigi Brunetti

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

مصطلحات موضوعية: Lipopolysaccharides, medicine.medical_specialty, Molecular biology, Science, Biopsy, medicine.medical_treatment, Anti-Inflammatory Agents, Prostaglandin, Inflammation, Growth Hormone-Releasing Hormone, Protective Agents, Dinoprostone, Article, Mice, chemistry.chemical_compound, In vivo, Internal medicine, Animals, Medicine, Intestinal Mucosa, Colitis, Nitrites, Multidisciplinary, L-Lactate Dehydrogenase, business.industry, Growth factor, Dextran Sulfate, Gastroenterology, Growth hormone–releasing hormone, medicine.disease, digestive system diseases, Disease Models, Animal, Endocrinology, chemistry, Cytokines, Tumor necrosis factor alpha, Inflammation Mediators, medicine.symptom, business, Biomarkers, Hormone

الوصف: Besides its metabolic and endocrine effects, growth hormone (GH)-releasing hormone (GHRH) is involved in the modulation of inflammation. Recently synthetized GHRH antagonist MIA-690 and MR-409, GHRH agonist, developed by us have shown potent pharmacological effects in various experimental paradigms. However, whether their administration modify resistance to chronic inflammatory stimuli in colon is still unknown. Ex vivo results demonstrated that MIA-690 and MR-409 inhibited production of pro-inflammatory and oxidative markers induced by lipopolysaccharide on isolated mouse colon specimens. In vivo, both MIA-690 and MR-409 have also been able to decrease the responsiveness to nociceptive stimulus, in hot plate test. Additionally, both peptides also induced a decreased sensitivity to acute and persistent inflammatory stimuli in male mice, in formalin test and dextran sodium sulfate (DSS)-induced colitis model, respectively. MIA-690 and MR-409 attenuate DSS-induced colitis with particular regard to clinical manifestations, histopathological damage and release of pro-inflammatory and oxidative markers in colon specimens. Respect to MR-409, MIA-690 showed higher efficacy in inhibiting prostaglandin (PG)E2, 8-iso-PGF2α and serotonin (5-HT) levels, as well as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and nitric oxide synthase gene expression in colon specimens of DSS-induced colitis. Furthermore, MIA-690 decreased serum insulin-like growth factor (IGF)-1 levels in mice DSS-treated, respect to MR-409. Thus, our findings highlight the protective effects of MIA-690 and MR-409 on inflammation stimuli. The higher antinflammatory and antioxidant activities observed with MIA-690 could be related to decreased serum IGF-1 levels.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a450469d8d65d1db85e9632944b7d05Test
https://doi.org/10.1038/s41598-021-81778-4Test

المؤلفون: Hideaki Tsuchiya, Daisuke Aoki, Hideaki Obara, Yusuke Matoba, Masatsugu Ema, Takashi Shiina, Takahiro Nakagawa, Hirohito Ishigaki, Kouji Banno, Mitsuru Murase, Yohei Yamada, Iori Itagaki, Hiroyuki Urano, Ikuo Kawamoto, Iori Kisu, Yoko Saiki, Kentaro Matsubara, Kenshi Nakagawa, Yohei Masugi, Chizuru Iwatani, Katsura Emoto, Kazumasa Ogasawara, Yojiro Kato

المصدر: Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)

مصطلحات موضوعية: 0301 basic medicine, Graft Rejection, Indocyanine Green, medicine.medical_specialty, Pathology, Time Factors, Reproductive disorders, medicine.medical_treatment, Uterus, lcsh:Medicine, Article, 03 medical and health sciences, chemistry.chemical_compound, Leukocyte Count, 0302 clinical medicine, Laparotomy, Internal medicine, Uterus transplantation, medicine, Animals, Transplantation, Homologous, lcsh:Science, Ultrasonography, Immunosuppression Therapy, Multidisciplinary, Hysterectomy, Hematology, L-Lactate Dehydrogenase, business.industry, lcsh:R, Optical Imaging, Immunosuppression, Transplantation, Experimental models of disease, Macaca fascicularis, 030104 developmental biology, medicine.anatomical_structure, C-Reactive Protein, chemistry, Infertility, lcsh:Q, Female, business, Indocyanine green, 030217 neurology & neurosurgery

الوصف: Uterus transplantation (UTx) is a potential option for women with uterine factor infertility to have a child. The clinical features indicating irreversible rejection of the uterus are unknown. In our experimental series of allogeneic UTx in cynomolgus macaques, six female macaques were retrospectively examined, which were unresponsive to treatment with immunosuppressants (i.e. irreversible rejection). Clinical features including general condition, hematology, uterine size, indocyanine green (ICG) fluorescence imaging by laparotomy, and histopathological findings of the removed uterus were evaluated. In all cases, general condition was good at the time of diagnosis of irreversible rejection and thereafter. Laboratory evaluation showed temporary increases in white blood cells, lactate dehydrogenase and C-reactive protein, then these levels tended to decrease gradually. In transabdominal ultrasonography, the uterus showed time-dependent shrinkage after transient swelling at the time of diagnosis of irreversible rejection. In laparotomy, a whitish transplanted uterus was observed and enhancement of the transplanted uterus was absent in ICG fluorescence imaging. Histopathological findings in each removed uterus showed hyalinized fibrosis, endometrial deficit, lymphocytic infiltration and vasculitis. These findings suggest that uterine transplantation rejection is not fatal, in contrast to rejection of life-supporting organs. Since the transplanted uterus with irreversible rejection atrophies naturally, hysterectomy may be unnecessary.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc8e91f1b6eb3d79276608c6a9122135Test
https://pubmed.ncbi.nlm.nih.gov/32807830Test

المؤلفون: Arvinder Kaur, Renu Bhardwaj, Kirpal Singh, Anket Sharma, Sunil Sharma, Owias Iqbal Dar

المصدر: Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)

مصطلحات موضوعية: 0301 basic medicine, Antioxidant, Carps, medicine.medical_treatment, Aspartate transaminase, lcsh:Medicine, Cosmetics, 010501 environmental sciences, Pharmacology, 01 natural sciences, Citric Acid, Article, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, Species Specificity, Lactate dehydrogenase, Malondialdehyde, medicine, Animals, Dicarboxylic Acids, lcsh:Science, 0105 earth and related environmental sciences, Multidisciplinary, biology, Dose-Response Relationship, Drug, Glutathione Disulfide, lcsh:R, Proteins, Glutathione, Oxidants, Triclosan, Enzymes, Environmental sciences, 030104 developmental biology, chemistry, Alanine transaminase, Toxicity, biology.protein, Anti-Infective Agents, Local, lcsh:Q, Zoology, Biomarkers, Water Pollutants, Chemical

الوصف: Accumulation, contents of protein, non-enzymatic antioxidant glutathione (GSH and GSSG), lipid peroxidation product (melondialdehyde-MDA) and organic acids (fumarate, succinate, malate and citrate), and activities of neurological (acetylcholinesterase-AChE), detoxification (glutathione S-transferase-GST) and metabolic (lactate dehydrogenase-LDH, aspartate transaminase-AST and alanine transaminase-ALT) enzymes were recorded in the hatchlings of Cyprinus carpio, Ctenopharyngodon idella, Labeo rohita and Cirrhinus mrigala after 7 and 14 days exposure and 10 days post exposure (recovery period) to sublethal concentrations (0.005, 0.01, 0.02 and 0.05 mg/L) of triclosan, a highly toxic and persistent biocide used in personal care products. Accumulation was maximum between 7–14 days at 0.01 mg/L for C. carpio and L. rohita but at 0.005 mg/L for C. idella and C. mrigala. No triclosan was observed at 0.005 mg/L in C. carpio and C. mrigala after recovery. Significant decline in protein, glutathione and acetylcholinesterase but increase in glutathione S-transferase, lactate dehydrogenase, aspartate transaminase, alanine transaminase, melondialdehyde and organic acids over control during exposure continued till the end of recovery period. Integrated biomarker response (IBR) analysis depicted higher star plot area for glutathione and glutathione S-transferase during initial 7 days of exposure, thereafter, during 7–14 days of exposure and the recovery period, higher star plot area was observed for acetylcholinesterase, aspartate transaminase, alanine transaminase and organic acids. Higher star plot area was observed for protein in all the species throughout the study. The study shows that L. rohita is most sensitive and glutathione, acetylcholinesterase, aspartate transaminase and alanine transaminase are the biomarkers for the toxicity of sublethal concentrations of TCS.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f074ad3783f1d90017f323923dd628cfTest
https://pubmed.ncbi.nlm.nih.gov/33057045Test

المؤلفون: Zbigniew Lewandowski, Marek Krawczyk, Michał Grąt, Krzysztof Zieniewicz, Piotr Krawczyk, Waldemar Patkowski, Michał Wasilewicz, Karolina Grąt, Jan Stypułkowski, Karolina M. Wronka

المصدر: Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
ResearcherID

مصطلحات موضوعية: Male, medicine.medical_specialty, Carcinoma, Hepatocellular, medicine.medical_treatment, Science, 030230 surgery, Milan criteria, Liver transplantation, Gastroenterology, Disease-Free Survival, 03 medical and health sciences, 0302 clinical medicine, Risk Factors, Internal medicine, medicine, Carcinoma, Living Donors, Humans, Aspartate Aminotransferases, Risk factor, Retrospective Studies, Multidisciplinary, biology, L-Lactate Dehydrogenase, business.industry, Hazard ratio, Liver Neoplasms, Retrospective cohort study, Alanine Transaminase, Middle Aged, medicine.disease, Liver Transplantation, Alanine transaminase, Hepatocellular carcinoma, Reperfusion Injury, biology.protein, Medicine, 030211 gastroenterology & hepatology, Female, Neoplasm Recurrence, Local, business

الوصف: This study aimed to evaluate the effects of ischemia-reperfusion injury (IRI) on the risk of hepatocellular carcinoma (HCC) recurrence after liver transplantation. Data of 195 patients were retrospectively analysed. Post-reperfusion aspartate (AST), alanine transaminase, and lactate dehydrogenase (LDH) levels were the primary measures of IRI. Tumour recurrence was the primary endpoint. Post-reperfusion AST was a continuous risk factor for tumour recurrence in patients within Milan criteria (p = 0.035), with an optimal cut-off of 1896 U/L. Recurrence-free survival of patients within Milan criteria and post-reperfusion AST of

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26013c42b1567a33e4b9a18a7112b970Test
https://pubmed.ncbi.nlm.nih.gov/29895820Test

المؤلفون: Dan Li, Shao-chin Lee, Lan Wang, Baozhen Li, Hans-Uwe Dahms, Jinhua Fan, Weixin Jing

المصدر: Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)

مصطلحات موضوعية: 0301 basic medicine, Antioxidant, Thioredoxin-Disulfide Reductase, medicine.medical_treatment, lcsh:Medicine, chemistry.chemical_element, Metal Nanoparticles, 02 engineering and technology, Pharmacology, Article, Antioxidants, Cell Line, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, Mice, Selenium, Sodium Selenite, Lactate dehydrogenase, medicine, Animals, Humans, lcsh:Science, Carbon Tetrachloride, chemistry.chemical_classification, Glutathione Peroxidase, Multidisciplinary, biology, Chemistry, Carbon Tetrachloride Poisoning, Glutathione peroxidase, Liver cell, lcsh:R, 021001 nanoscience & nanotechnology, 030104 developmental biology, Catalase, biology.protein, Alkaline phosphatase, lcsh:Q, Chemical and Drug Induced Liver Injury, 0210 nano-technology

الوصف: Elemental selenium nanoparticles (SeNPs) have multiple biological activities. In this study, we investigated the protective effects of biogenic SeNPs (BioSeNPs) on CCl4-induced liver damage in mice. The results showed that: (i) when compared to sodium selenite (SS), BioSeNPs has a similar tissue distribution after intragastrical administration to mice; (ii) BioSeNPs and SS showed comparable efficacy in increasing the activities of glutathione peroxidase and thioredoxin reductase in liver cell lines, mice blood and liver; (iii) pretreatment with BioSeNPs inhibiting the elevation of activities of various enzymes significantly which included aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase and liver lipid peroxide (p 4-treated mice; (iv) activities of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) were significantly increased (p 4-treated mice; (v) histopathological damages in the liver from CCl4-treated mice were ameliorated by a pretreatment with BioSeNPs. In conclusion, these results have shown that BioSeNPs is able to protect the liver from CCl4-induced hepatic damage via increasing the antioxidant capacity and inhibiting oxidative damage. BioSeNPs may have the potential to be used as a trace element food supplement inducing antioxidant bioactivities.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::085aab269ed8c3066725747071db11fcTest
http://europepmc.org/articles/PMC5688085Test