التفاصيل البيبلوغرافية
| العنوان: |
Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence. |
| المؤلفون: |
Nour, Islam, Hanif, Atif, Alanazi, Ibrahim O., Al-Ashkar, Ibrahim, Alhetheel, Abdulkarim, Eifan, Saleh |
| المصدر: |
Scientific Reports; 6/9/2021, Vol. 11 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
FECAL contamination, ROTAVIRUSES, HYDROPHOBIC interactions, GENOTYPES, PROLINE |
| مستخلص: |
The routine evaluation of water environments is necessary to manage enteric virus-mediated fecal contamination and the possible emergence of novel variants. Here, we detected human rotavirus A (HRVA) circulating in two wastewater treatment plants, two lakes, irrigation water and a wastewater landfill located in Riyadh. VP7-derived surface protein sequences were assessed by phylogenetic analyses and inspection of thermotolerance-mediated secondary structure and seasonal variation. HRVA was most prevalent at An-Nazim wastewater landfill (AN-WWLF; 63.89%). Phylogenetic analyzes revealed the predominance of HRVA G2 lineage for the first time in Saudi Arabia. Moreover, a single HRVA sequence (2B64I-ANLF3/2018) was recovered at 45 °C from AN-WWLF; secondary structure prediction indicated that this sequence was thermotolerant with a high hydrophobicity, an absence of Ramachandran outliers, and a higher content of proline patches on the protein surface. Varied relationships were significantly observed between sampling areas influenced by temperature ranges (p < 0.05). HRVA prevalence was influenced by seasonal variations, favoring moderate temperatures in late autumn and early winter in all locations. However, a significant temperature impact was detected in Wadi-Hanifah Lake (p = 0.01). Our study extends the knowledge of currently circulating HRVA genotypes, and indicates the probable emergence of thermotolerant strains and seasonally mediated HRVA prevalence. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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