المؤلفون: Li, Yan^1,2 (AUTHOR) liy@sderi.cn, Hua, Dongliang^1,2 (AUTHOR), Xu, Haipeng^1,2 (AUTHOR), Jin, Fuqiang^1,2 (AUTHOR), Zhao, Yuxiao^1,2 (AUTHOR), Chen, Lei^1,2 (AUTHOR), Zhao, Baofeng^1,2 (AUTHOR), Rosendahl, Lasse A.³ (AUTHOR), Zhu, Zhe^1,4 (AUTHOR) zhuzhe@tjut.edu.cn

المصدر: Science of the Total Environment. Oct2022, Vol. 843, pN.PAG-N.PAG. 1p.

المؤلفون: Hua, Dongliang^1,2 (AUTHOR), Fan, Qingwen¹ (AUTHOR), Zhao, Yuxiao^1,2 (AUTHOR), Xu, Haipeng^1,2 (AUTHOR), Chen, Lei^1,2 (AUTHOR), Li, Yan^1,2,3 (AUTHOR) liy@sderi.cn

المصدر: Science of the Total Environment. Oct2020, Vol. 739, pN.PAG-N.PAG. 1p.

مستخلص: Anaerobic digestion (AD) of wood vinegar wastewater (WVWW) has considerable potential in energy recovery and sustainable development. WVWW contains abundant acetic acid and some refractory organics. Therefore, the batch and continuous AD of WVWW were investigated. The threshold value of the inhibitory concentration was obtained at a chemical oxygen demand (COD) of 4 g/L in batch AD. Three-dimensional electrolysis was adopted to improve the biodegradability of WVWW, and a reduction in the inhibitory rate from 38.2% to 4.9% and an increase in methane production by 53.8% were observed. The up-flow anaerobic sludge blanket reactor achieved an efficient conversion of methane at an organic loading rate (OLR) of <8.58 g COD/L·d. However, the OLR of 10.01 g COD/L·d decreased the methane production from 350.6 to 42.5 mL CH 4 /g COD fed. Aminicenantales , Acetobacterium , Anaerolineae , and SBR1031 were the dominant bacterial genera in continuous AD. Fewer genera with similar classifications were detected in the batch AD. In the archaea community, acetotrophic methanogens (Methanosaeta) dominated and increased continuously with increasing OLR. Microbial analysis revealed that toxic substances affected bacterial diversity and promoted the enrichment of Intestinimonas , Syntropobacter , and Propionicimonas at high OLRs. The continuous AD was most suitable for the energy recovery from WVWW. Unlabelled Image • The maximum OLR of continuous AD was 8.58 g COD/L·d. • Inhibitory rate was reduced from 38.2% to 4.9% by 3D-electrolysis at 4 g COD/L. • Methanogenic activity was transiting from non-inhibition to inhibition at 8.58 g COD/L·d. • Bacteria and archaea showed significant shift with the gradient OLRs of WV-WW. [ABSTRACT FROM AUTHOR]

المؤلفون: Li, Yan^1,2,3 (AUTHOR), Xu, Haipeng^2,3 (AUTHOR), Hua, Dongliang^2,3 (AUTHOR), Zhao, Baofeng^2,3 (AUTHOR), Mu, Hui^2,3 (AUTHOR), Jin, Fuqiang^2,3 (AUTHOR), Meng, Guangfan^2,3 (AUTHOR), Fang, Xu¹ (AUTHOR) fangxu@sdu.edu.cn

المصدر: Science of the Total Environment. Jan2020, Vol. 699, pN.PAG-N.PAG. 1p.

مستخلص: • Two-phase anaerobic digestion of lignocellulosic hydrolysate was proposed. • The acidogenic performance of corn straw hydrolysate was illustrated. • Anaerobic and aerobic sludge could be both utilized as the inocula for acidogenesis. • The bacterial community diversities of inocula and digestates were investigated. Biogas production from lignocellulosic hydrolysate is of great potential for lignocellulosic materials. Two-phase anaerobic digestion was proposed in this study. Acidogenic fermentation was carried out with corn straw hydrolysate as feedstock for volatile fatty acids (VFAs) production. Using anaerobic sludge (AnS), different inoculum to substrate ratios (ISRs) of 0.5:1, 1:1 and 2:1 were investigated. The highest VFAs yield was obtained at ISR of 0.5:1.VFAs composition analysis showed that butyric acid was the predominant acid, followed by acetic acid and propionic acid. The effects of AnS and aerobic sludge (AeS) on the acidogenic performance of hydrolysate were compared. The optimum VFA yields were 0.38 g/g COD-added for AnS and 0.32 g/g COD-added for AeS with HRT of 5 d, respectively. The bacterial diversities of inocula and digestates were analyzed by high-throughput sequencing. Two origins of inocula had distinct bacterial structures, but they did share core communities that included Firmicutes, Chloroflexi, Proteobacteria and Bacteroidetes at phylum level. The bacterial communities of both digestates changed significantly as compared with those in inoculum. Firmicutes was absolutely dominant in all the bacterial species. Therefore, the AeS could be an option as the acidogenic inoculum. The microbial information will be beneficial for the enrichment and acclimatization of microbes. In methanogenic process, VFAs obtained in acidogenic stage could be efficiently converted into methane. The ultimate methane yield at organic loading rate (OLR) of 8 g/L·d could reach 290 mL CH 4 /g COD-added and 279 mL CH 4 /g COD-added for AnS and AeS acidified digestate. Two-phase anaerobic digestion was proved to be suitable for bioconversion of lignocellulosic hydrolysate into biogas. [ABSTRACT FROM AUTHOR]

المؤلفون: Xu, Haipeng^1,2 (AUTHOR), Li, Yan² (AUTHOR), Hua, Dongliang² (AUTHOR), Mu, Hui² (AUTHOR), Zhao, Yuxiao² (AUTHOR), Chen, Guanyi¹ (AUTHOR) chen@tju.edu.cn

المصدر: Science of the Total Environment. Dec2019, Vol. 694, pN.PAG-N.PAG. 1p.

مستخلص: This work evaluated the methane potential and methane production rate of the stem bark (SB), stem pith (SP), and leaves (LV) of corn stover from batch anaerobic digestion. The obtained cumulative methane potential and the hydrolysis kinetics constant were 0.201, 0.214, and 0.199 L g−1 VS (volatile solids) and 0.090, 0.149, and 0.227 d−1 for SB, SP, and LV, respectively. The chemical composition and the crystalline structure of the substrates as well as their changes during the anaerobic digestion were inspected, and their impacts on the characteristics of methane production were assessed. The methane production rate correlated positively with the hemicellulose and soluble compounds content and negatively with the cellulose and lignin content, but the degradation rates of hemicellulose and cellulose in the specific substrate were complex and comparable. The methane production has limit correlation with the crystalline structure of the substrates. Microbial community structure was analyzed to elucidate functional microorganism contributing to methane production of different substrate. The abundance of Bacteroidetes and Firmicutes was most affected by the substrate, and positively related to methane yields. Unlabelled Image • The methane yields and hydrolysis kinetic constants of SB, SP, and LV were evaluated. • The chemical and physical changes of the substrates during digestion were inspected. • Correlation was established between chemical changes and methane production. • The crystalline structure of cellulose had limit effect to anaerobic digestion of SB. • The abundance of Bacteroidetes and Firmicutes was positively related to methane yields. [ABSTRACT FROM AUTHOR]