دورية أكاديمية
Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration
| العنوان: | Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration |
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| المؤلفون: | Gerber, T., Murawala, P., Knapp, D., Masselink, W., Schuez, M., Hermann, S., Gac-Santel, M., Nowoshilow, S., Kageyama, J., Khattak, S., Currie, J., Camp, J., Tanaka, E., Treutlein, B. |
| المصدر: | Science |
| سنة النشر: | 2018 |
| المجموعة: | Max Planck Society: MPG.PuRe |
| الوصف: | How the axolotl makes a new limb Unlike most vertebrate limbs, the axolotl limb regenerates the skeleton after amputation. Dermal and interstitial fibroblasts have been thought to provide sources for skeletal regeneration, but it has been unclear whether preexisting stem cells or dedifferentiation of fibroblasts formed the blastema. Gerber et al. developed transgenic reporter animals to compare periskeletal cell and fibroblast contributions to regeneration. Callus-forming periskeletal cells extended existing bone, but fibroblasts built new limb segments. Single-cell transcriptomics and Brainbow-based lineage tracing revealed the lack of a preexisting stem cell. Instead, the heterogeneous population of fibroblasts lost their adult features to form a multipotent skeletal progenitor expressing the embryonic limb program. Science, this issue p. eaaq0681 Structured Abstract INTRODUCTIONAxolotls (Ambystoma mexicanum) and other salamanders are the only tetrapods that can regenerate whole limbs. During this complex process, changes in gene expression regulate the outgrowth of a new appendage, but how injury induces limb cells to form regenerative progenitors that differentiate into diverse cell fates is poorly understood. Tracking and molecular profiling of individual cells during limb regeneration would resolve distinct differentiation pathways and provide clues for how cells convert from a mature resting state into regenerative cell lineages. RATIONALEAxolotl limbs are composed of many different cell types originating from neural, myogenic, epidermal, and connective tissue (CT) lineages. Upon limb amputation, cells from nearby the amputation plane accumulate in a distinctive tissue called the blastema, which serves as a progenitor cell source to build the new limb. Transgenic axolotl strains in which descendants of distinct adult cell types can be labeled, tracked, and isolated during the regenerative process provide an opportunity to understand how particular cell lineages progress during blastema formation and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://hdl.handle.net/21.11116/0000-0002-9E6C-4Test |
| الإتاحة: | https://doi.org/10.1126/science.aaq0681Test http://hdl.handle.net/21.11116/0000-0002-9E6C-4Test |
| رقم الانضمام: | edsbas.AB0584D3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |