التفاصيل البيبلوغرافية
| العنوان: |
Genomic Organization and Promoter Analysis of the Gene ifngr2 Encoding the Second Chain of the Mouse Interferon-ϒ Receptor. |
| المؤلفون: |
Ebensperger, C., Rhee, S., Muthukumaran, G., Lembo, D., Donnelly, R., Pestka, S., Dembic, Z. |
| المصدر: |
Scandinavian Journal of Immunology; Dec1996, Vol. 44 Issue 6, p599-606, 8p, 5 Diagrams, 1 Graph |
| مصطلحات موضوعية: |
CLONE cells, CELL culture, DNA, TRANSCRIPTION factors, PROMOTERS (Genetics) |
| مستخلص: |
A clone containing the gene ifngr2 for the second chain (IFN-γR2) of the mouse interferon γ receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5′-flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP-2, NF1, EGR and NFκB. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
