CD4+CD25+ regulatory T cells from MRL/lpr mice were functionally more active in vitro but did not prevent spontaneous as well as adriamycin-induced nephropathy in vivo
| العنوان: | CD4+CD25+ regulatory T cells from MRL/lpr mice were functionally more active in vitro but did not prevent spontaneous as well as adriamycin-induced nephropathy in vivo |
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| المؤلفون: | Ju Young Seoh, Woosung Lim, Hyung-Ran Kim, Jeong-Hae Kie, Byung In Moon, Seung Cheol Kim |
| المصدر: | Rheumatology. 51:1357-1367 |
| بيانات النشر: | Oxford University Press (OUP), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Male, Mice, Inbred MRL lpr, CD11c, chemical and pharmacologic phenomena, In Vitro Techniques, Carboxyfluorescein diacetate succinimidyl ester, Lymphocyte Activation, urologic and male genital diseases, Severity of Illness Index, T-Lymphocytes, Regulatory, Pathogenesis, Mice, chemistry.chemical_compound, Rheumatology, immune system diseases, In vivo, medicine, Animals, Pharmacology (medical), IL-2 receptor, skin and connective tissue diseases, Cells, Cultured, Kidney, Nephritis, Systemic lupus erythematosus, business.industry, Interleukin-2 Receptor alpha Subunit, hemic and immune systems, medicine.disease, In vitro, medicine.anatomical_structure, chemistry, Doxorubicin, Immunology, business |
| الوصف: | Objective. The frequency and function of Tregs are important in the pathogenesis of SLE. Nonetheless, the function of Tregs is still controversial in SLE patients and lupus mouse models. In the present study, we investigated the suppressive function of Tregs from MRL/lpr mice in vitro and in vivo by using an alternative quantitative assay. Methods. We assessed the suppressive function of CD4 + CD25 + Tregs, the proliferative activity of CD4 + CD25 effector T cells (Teffs) and the feeder activity of CD11c + dendritic cells (DCs), isolated from the spleens of MRL/lpr mice and wild-type (WT) MRL/+ mice, by carboxyfluorescein diacetate succinimidyl ester dilution assay stimulated with two distinct types of signals, weak and strong. In order to assess the protective function of Tregs from an immune-mediated disease in vivo, we induced renal damage by injecting adriamycin (ADN) into the mice. Results. The in vitro assay showed enhanced suppressive activity of Tregs and feeder activity of DCs, but far less proliferative activity of Teffs from MRL/lpr mice, compared with those from the WT mice. The in vivo study showed more severe ADN-induced nephropathy in MRL/lpr mice than in the WT mice, while mild interstitial nephritis had already begun spontaneously by 16 weeks in MRL/lpr mice. Conclusion. It was suggested that Tregs from MRL/lpr mice were functionally competent and intrinsically more active in vitro, but they were not capable of preventing the ADN-induced as well as the spontaneously developing nephropathy in vivo. |
| تدمد: | 1462-0332 1462-0324 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2588d1c5de7f74bd604fb51a38705f9eTest https://doi.org/10.1093/rheumatology/kes058Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2588d1c5de7f74bd604fb51a38705f9e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14620332 14620324 |
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