Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-β knock-out mice
| العنوان: | Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-β knock-out mice |
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| المؤلفون: | Shohreh Issazadeh-Navikas, Ingrid Teige, Victor Matheu, Alexandra Treschow, Vaidrius Navikas |
| المصدر: | Respiratory Research, Vol 6, Iss 1, p 25 (2005) Respiratory Research |
| بيانات النشر: | BMC, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Allergy, CpG motifs, Ovalbumin, CpG Oligodeoxynucleotide, Arthritis, knockout, Inflammation, lung, Mice, Adjuvants, Immunologic, Respiratory Hypersensitivity, medicine, Animals, eosinophil, IFN-γ, IFN-β, Mice, Knockout, lcsh:RC705-779, biology, Research, Th1-response, hemic and immune systems, Interferon-beta, lcsh:Diseases of the respiratory system, Eosinophil, respiratory system, asthma, medicine.disease, allergy, Disease Models, Animal, Treatment Outcome, medicine.anatomical_structure, Oligodeoxyribonucleotides, CpG site, arthritis, inflammation, Immunology, Knockout mouse, biology.protein, Cytokines, CpG Islands, Female, medicine.symptom, synovitis |
| الوصف: | Background CpG oligodeoxynucleotides (CpG-ODN) are capable of inducing high amounts of type I IFNs with many immunomodulatory properties. Furthermore, type-I IFNs have been proposed to play a key role in mediating effects of CpG-ODN. The precise role of IFN-β in the immunomodulatory effects of CpG-ODN is not known. Objective Here, we aimed to elucidate the role of IFN-β in the anti-allergic effect of CpG motifs. Methods We assessed the immune response in OVA-primed/OVA-challenged IFN-β knockout (-/-) mice compared to wild type (WT) control, after intranasal and systemic treatment with synthetic CpG motifs. Results Vaccination with CpG-ODN reduced the number of cells in airways of OVA-sensitized WT but not IFN-β-/- mice. Although airway eosinophilia was reduced in both treated groups, they were significantly higher in IFN-β-/- mice. Other inflammatory cells, such as lymphocytes and macrophages were enhanced in airways by CpG treatment in IFN-β-/- mice. The ratio of IFN-γ/IL-4 cytokines in airways was significantly skewed to a Th1 response in WT compared to IFN-β-/- group. In contrast, IL-4 and IgE were reduced with no differences between groups. Ag-specific T-cell proliferation, Th1-cytokines such as IFN-γ, IL-2 and also IL-12 were significantly lower in IFN-β-/- mice. Surprisingly, we discovered that intranasal treatment of mice with CpG-ODN results in mild synovitis particularly in IFN-β-/- mice. Conclusion Our results indicate that induction of Th1 response by therapy with CpG-ODN is only slightly and partially dependent on IFN-β, while IFN-β is not an absolute requirement for suppression of airway eosinophilia and IgE. Furthermore, our finding of mild synovitis is a warning for possible negative effects of CpG-ODN vaccination. |
| اللغة: | English |
| تدمد: | 1465-9921 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::510fe49d4d0ccb6b941eb095126c7d3cTest http://respiratory-research.com/content/6/1/25Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....510fe49d4d0ccb6b941eb095126c7d3c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14659921 |
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