The cationic amino acid transporter 2 is induced in inflammatory lung models and regulates lung fibrosis
| العنوان: | The cationic amino acid transporter 2 is induced in inflammatory lung models and regulates lung fibrosis |
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| المؤلفون: | Kathryn A Niese, Marc E. Rothenberg, Nives Zimmermann, Lesley G. Ellies, Monica G. Chiaramonte |
| المصدر: | Respiratory Research, Vol 11, Iss 1, p 87 (2010) Respiratory Research Niese, Kathryn A; Chiaramonte, Monica G; Ellies, Lesley G; Rothenberg, Marc E; & Zimmermann, Nives. (2010). The cationic amino acid transporter 2 is induced in inflammatory lung models and regulates lung fibrosis. Respiratory Research, 11(1), 87. doi: http://dx.doi.org/10.1186/1465-9921-11-87Test. Retrieved from: http://www.escholarship.org/uc/item/08r3r3gmTest |
| بيانات النشر: | BMC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Arginine, Ovalbumin, Pulmonary Fibrosis, Inflammation, Mice, Transgenic, Biology, 03 medical and health sciences, chemistry.chemical_compound, Bleomycin, Mice, 0302 clinical medicine, Pulmonary fibrosis, medicine, Animals, Amino acid transporter, RNA, Messenger, Lung, Interleukin 4, 030304 developmental biology, Mice, Knockout, lcsh:RC705-779, 0303 health sciences, Arginine transport, Arginase, Macrophages, Research, lcsh:Diseases of the respiratory system, Ornithine, respiratory system, medicine.disease, Molecular biology, Asthma, 3. Good health, respiratory tract diseases, Up-Regulation, Mice, Inbred C57BL, Disease Models, Animal, chemistry, Amino Acid Transport Systems, Basic, Cytokines, Collagen, Interleukin-4, medicine.symptom, Inflammation Mediators, STAT6 Transcription Factor, 030215 immunology |
| الوصف: | Background Arginine is an amino acid that serves as a substrate for the enzymes nitric oxide synthase (NOS) and arginase, leading to synthesis of NO and ornithine, respectively. As such, arginine has the potential to influence diverse fundamental processes in the lung. Methods We used mice deficient in cationic amino acid transporter (CAT) 2 in models of allergic airway inflammation and pulmonary fibrosis. Results We report that the arginine transport protein CAT2 was over-expressed in the lung during the induction of allergic airway inflammation. Furthermore, CAT2 mRNA was strongly induced by transgenically over-expressed IL-4, and allergen-induced expression was dependent upon signal-transducer-and-activator-of-transcription (STAT) 6. In situ mRNA hybridization demonstrated marked staining of CAT2, predominantly in scattered mononuclear cells. Analysis of allergic airway inflammation and bleomycin-induced inflammation in CAT2-deficient mice revealed that while inflammation was independent of CAT2 expression, bleomycin-induced fibrosis was dependent upon CAT2. Mechanistic analysis revealed that arginase activity in macrophages was partly dependent on CAT2. Conclusion Taken together, these results identify CAT2 as a regulator of fibrotic responses in the lung. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1465-9921 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff488e09613d0836ca395abf78fca43bTest http://respiratory-research.com/content/11/1/87Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ff488e09613d0836ca395abf78fca43b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14659921 |
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