المؤلفون: Hao Zheng, Guangzhi Tong, Bao-Yang Ruan, Xiao-Min Liu, Wu Tong, Hai Yu, Guoxin Li, Peng Zhang, Xiu-Hui Wang, Yan-Jun Zhou, Xiao-Qian Gong, Qi Wang, Shan Tongling

المصدر: Research in Veterinary Science. 117:54-56

مصطلحات موضوعية: 0301 basic medicine, animal diseases, viruses, Mutant, Virulence, Biology, Virus Replication, medicine.disease_cause, H5N1 genetic structure, Virus, Pathogenesis, Mice, Viral Proteins, 03 medical and health sciences, Orthomyxoviridae Infections, medicine, Animals, Cells, Cultured, Mutation, General Veterinary, Influenza A Virus, H3N2 Subtype, virus diseases, Antigenic shift, biochemical phenomena, metabolism, and nutrition, RNA-Dependent RNA Polymerase, Virology, respiratory tract diseases, 030104 developmental biology, Viral replication

الوصف: PB2-627K is an important amino acid that determines the virulence of some influenza A viruses. However, it has not been experimentally investigated in the H3N2 swine influenza virus. To explore the potential role of PB2-K627E substitution in H3N2 swine influenza virus, the growth properties and pathogenicity between H3N2 swine influenza virus and its PB2-K627E mutant were compared. For the first time, our results showed that PB2-K627E mutation attenuates H3N2 swine influenza virus in mammalian cells and in mice, suggesting that PB2-627K is required for viral replication and pathogenicity of H3N2 swine influenza virus.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c4157322119d01df85eb3a51ade5865Test
https://doi.org/10.1016/j.rvsc.2017.11.006Test

المؤلفون: Guangzhi Tong, Liping Yan, Hai Yu, Guoxin Li, Shouping Hu, Qiang Zhang, Yan-Jun Zhou, Zhi-Jun Tian

المصدر: Research in Veterinary Science. 88:345-351

مصطلحات موضوعية: Enzyme-Linked Immunosorbent Assay, Biology, Recombinant virus, medicine.disease_cause, Virus, DNA vaccination, law.invention, Interferon-gamma, Mice, Orthomyxoviridae Infections, Neutralization Tests, law, Pseudorabies Vaccines, Vaccines, DNA, Influenza A virus, medicine, Animals, Mice, Inbred BALB C, General Veterinary, Influenza A Virus, H3N2 Subtype, Immunogenicity, Vector vaccine, Virology, Recombinant Proteins, Hemagglutinins, Complement C3d, Humoral immunity, Recombinant DNA, Female, Interleukin-4

الوصف: DNA and recombinant virus vaccines against swine influenza virus (SIV) have been pursued with promising results, but induce poor immunogenicity. This study evaluated the effects of a vaccine regimen in mice including priming with three DNA vaccines expressing soluble HA (sHA), complete HA (tmHA), or sHA fused with three copies murine C3d (sHA-mC3d3) and boosting with recombinant pseudorabies virus expressing HA (rPRV-HA). Immune responses were monitored by ELISA, HI assays, and virus neutralization. Protective efficacy was evaluated by virus isolation from lungs, distribution in tissues, and pathology following challenge with H3N2 SIV. Priming with sHA-mC3d3 and boosting with rPRV-HA induced higher levels of HA-specific antibodies and yielded the most effective protection. This finding implied that priming with a DNA vaccine expressing C3d fused with antigen and boosting with a recombinant vector vaccine is an effective way to induce protective humoral immunity and prevent some infectious diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::900b9bf041a2598887c3b58b65ff0488Test
https://doi.org/10.1016/j.rvsc.2009.09.005Test

المؤلفون: Tian-Qiang Liu, Hai Yu, Guangzhi Tong, Yuan-Yuan Jin, Zhi-Jun Tian, Shouping Hu, Guoxin Li, Shao-Hua Hou, Yan-Jun Zhou

المصدر: Research in veterinary science. 86(3)

مصطلحات موضوعية: Hemagglutination, Swine, animal diseases, viruses, Recombinant Fusion Proteins, Molecular Sequence Data, Hemagglutinin (influenza), chemical and pharmacologic phenomena, Enzyme-Linked Immunosorbent Assay, Biology, medicine.disease_cause, Polymerase Chain Reaction, Virus, DNA vaccination, Mice, Influenza A Virus, H1N1 Subtype, Orthomyxoviridae Infections, Influenza A virus, medicine, Vaccines, DNA, Animals, Cloning, Molecular, DNA Primers, B-Lymphocytes, Immunity, Cellular, Hemagglutination assay, General Veterinary, Base Sequence, Influenza A Virus, H3N2 Subtype, Antibody titer, Immunity, virus diseases, Dendritic Cells, Hemagglutination Inhibition Tests, Virology, Hemagglutinins, Complement C3d, Antibody Formation, biology.protein, Receptors, Complement 3d, Antibody

الوصف: H1N1 and H3N2 are the dominant subtypes causing swine influenza in China and other countries. It is important to develop effective vaccines against both H1N1 and H3N2 subtypes of swine influenza virus (SIV). We examined the effects of a DNA vaccine expressing an influenza HA fused to three copies of murine complement C3d in mice. Plasmids encoding soluble HA (sHA), complete HA (tmHA), or a soluble fused form of HA (sHA-mC3d3) were constructed from the H3N2 subtype of SIV. The immune response was monitored by an enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition (HI) assays, and virus neutralization tests. Analysis of antibody titers indicated that immunization with HA-mC3d3 resulted in higher titers of anti-HA antibodies and higher antibody affinities, compared with serum from mice immunized with sHA or tmHA. Furthermore, the C3d fusion increased the Th2-biased immune response, by inducing IL-4 production. Splenocytes from mice immunized with sHA-mC3d3 produced about three-fold more IL-4 than did splenocytes from mice immunized with sHA or tmHA. Seven days post-challenge with homologous virus (H3N2), no virus was isolated from the mice immunized with HA-expressing plasmids. However, 10 days post-challenge with heterologous virus (H1N1), only mice immunized with sHA-mC3d3 had no virus or microscopic lesions in the kidneys and cerebrum. In conclusion, C3d enhanced antibody responses to hemagglutinin and protective immunity against SIV of different subtypes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d0aca8f7c6e20c126adfb78406cb978Test
https://pubmed.ncbi.nlm.nih.gov/19010505Test

المؤلفون: Guangzhi Tong, Wu Tong, Shen Yang, Liwei Li, Lingxue Yu, Hao Zheng, Qinfeng Huang, Zehui Qu, Fei Gao, Yifeng Jiang, Yan-Jun Zhou

المصدر: Research in Veterinary Science

مصطلحات موضوعية: Gene Expression Regulation, Viral, 0301 basic medicine, Swine, viruses, Porcine Reproductive and Respiratory Syndrome, Biology, Virus Replication, Virus, Cell Line, 03 medical and health sciences, Macrophages, Alveolar, Gene expression, Animals, Porcine respiratory and reproductive syndrome virus, Luciferase, Luciferases, Gene, General Veterinary, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, Virology, Molecular biology, Recombinant Proteins, 030104 developmental biology, Viral replication, Cell culture, Regulatory sequence

الوصف: Porcine reproductive and respiratory syndrome virus (PRRSV) has a condensed single-stranded positive-sense RNA genome that contains several overlapping regions. The transcription regulatory sequence (TRS) is the important cis-acting element participating in PRRSV discontinuous transcription process. Based on reverse genetic system of type 2 highly pathogenic PRRSV cell-passage attenuated strain pHuN4-F112, firefly luciferase or Renilla luciferase genes were inserted between ORF1b and ORF2. An extra TRS6 was embedded behind the foreign luciferase genes. pA-Fluc and pA-Rluc were constructed and successfully rescued in MARC-145 cells. The phenotypical characteristics of the progeny virus were indistinguishable from those of vHuN4-F112 and were genetically stable for at least 25 cell passages. Mutant virus-infected cells were lysed at different time points to assess luciferase activities and measure foreign gene expression levels. The results showed identical variations in the luciferase activities of the recombinants in MARC-145 cells, indicating that they were suitable for monitoring viral propagation in PRRSV-permissive cell cultures. They were also used to infect pulmonary alveolar macrophages, which yielded similar variations in luciferase activities. Therefore, vA-Fluc and vA-Rluc present powerful new tools to monitor PRRSV propagation in both passaged and target cells.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a54afbd3d8912e05efde8c37f136b5efTest