Hypoxia and its possible relationship with endometrial receptivity in adenomyosis: a preliminary study
| العنوان: | Hypoxia and its possible relationship with endometrial receptivity in adenomyosis: a preliminary study |
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| المؤلفون: | Yijuan Sun, Song Guo, Ruihuan Gu, Di Zhang, Qian Zhang, Binghui Sun, Xiaowei Lu |
| المصدر: | Reproductive Biology and Endocrinology : RB&E Reproductive Biology and Endocrinology, Vol 19, Iss 1, Pp 1-8 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Gene Expression, Endometrium, Mice, 0302 clinical medicine, Endocrinology, Basic Helix-Loop-Helix Transcription Factors, lcsh:Reproduction, Sulfones, Hypoxia, 030219 obstetrics & reproductive medicine, Female infertility, Obstetrics and Gynecology, Immunohistochemistry, Blot, Endometrial receptivity, Indans, Female, Adenomyosis, medicine.drug, Adult, Infertility, lcsh:QH471-489, Hypoxia-inducible factor-2α, lcsh:Gynecology and obstetrics, Andrology, 03 medical and health sciences, In vivo, medicine, Animals, Humans, Embryo Implantation, lcsh:RG1-991, Retrospective Studies, Homeodomain Proteins, business.industry, Research, Antagonist, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Disease Models, Animal, Homeobox A10 Proteins, 030104 developmental biology, Animals, Newborn, Reproductive Medicine, PT2399, business, Tamoxifen, Developmental Biology |
| الوصف: | Background Adenomyosis (AM) is an important cause of female infertility. However, the underlying mechanism remains unclear. This report describes a preliminary study of hypoxia and its possible association with endometrial receptivity in AM. Methods The study was divided into in vitro and in vivo experiments. In vitro, expression levels of the endometrial receptivity markers HOXA10 and HOXA11 in the implantation period were examined using real-time PCR and western blotting. Endometrial expression of hypoxia-inducible factor (HIF)-1α, HIF-2α, and HIF-3α was determined using immunohistochemistry. In vivo, using an AM mouse model established by oral administration of tamoxifen, we inhibited expression of HIF-2α using an HIF-2α antagonist (PT2399; 30 mg/kg body weight, twice daily by oral gavage for 2 days) and then examined expression levels of Hoxa10 and Hoxa11 using real-time PCR and western blotting. Results Endometrial mRNA and protein expression levels of HOXA10 and HOXA11 were significantly lower in patients with AM than in control patients. Expression of HIF-2α was significantly higher in the AM group than in the control group, whereas that of HIF-1α and HIF-3α was equivalent in both groups. In vivo analysis showed that administration of the HIF-2α antagonist resulted in increased expression of Hoxa10 and Hoxa11 at both the mRNA and protein levels in AM model mice. Conclusions HIF-2α overexpression may be one reason for decreased endometrial receptivity in AM. The current findings provide insight into HIF-2α-mediated AM-related infertility and suggest that PT2399 has potential as a treatment for AM. Trial registration This trial was retrospectively registered. |
| تدمد: | 1477-7827 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f930e6c5f2e2f1173952862cb5df508Test https://doi.org/10.1186/s12958-020-00692-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7f930e6c5f2e2f1173952862cb5df508 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14777827 |
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