Homology between cattle bull sperm and bacterial antigenic proteins viz a viz possible role in immunological infertility
| العنوان: | Homology between cattle bull sperm and bacterial antigenic proteins viz a viz possible role in immunological infertility |
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| المؤلفون: | Ranjna S. Cheema, Ankit Kumar Ahuja |
| المصدر: | Reproduction in Domestic Animals. 53:1530-1538 |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Staphylococcus, Bacillus, Dehydrogenase, medicine.disease_cause, Antibodies, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Bacterial Proteins, Capacitation, Escherichia coli, medicine, Animals, Antigens, Bacterial, 030219 obstetrics & reproductive medicine, Chemistry, Malate dehydrogenase 2, Acrosin, Spermatozoa, Sperm, Molecular biology, 030104 developmental biology, Immunoglobulin G, Infertility, Cattle, Female, Animal Science and Zoology, Bacterial antigen, Bacterial outer membrane, Sperm Capacitation, Biotechnology |
| الوصف: | This study was carried out to investigate the possible presence of identical sperm and bacterial antigens which may cause similar antisperm antibody production leading to lower fertility. Cross-reactive antigens of cattle bull spermatozoa and different bacteria including Escherichia coli (E. coli), Bacillus sp., and Staphylococcus sp. were characterized by immunoblotting and mass fingerprinting. Significant cross-reactivity was obtained for 75, 72, 44, 40, 33, 30, 25, 18, 14 kDa proteins with purified IgG of calves, heifers and cows between spermatozoa and the studied bacteria. Significantly (p < 0.05) matched cross-reactive 40/33/30 kDa sperm, 33 kDa Staphylococcus sp/Bacillus sp and 40/25 kDa E. coli proteins were analyzed. Mass fingerprinting of 40/33/30 kDa (spermatozoa); 40/25 kDa (E. coli) and 33 kDa (Bacillus/Staphylococcus) proteins revealed their matching with vitellogenin-1-like/mitochondrial malate dehydrogenase 2, NAD/acrosin-binding protein isoform XI; outer membrane insertion signal domain/spore coat protein and glyceraldehyde-3-phosphate dehydrogenase, respectively. Acrosin-binding protein isoform X1 and mitochondrial malate dehydrogenase 2, NAD contributes to the capacitation of spermatozoa. Spore coat protein; glyceraldehyde-3-phosphate dehydrogenase of E. coli; Bacillus/Staphylococcus are 37.6% and 39.01% identical to acrosin-binding protein isoform X1; mitochondrial malate dehydrogenase 2, NAD of cattle bull spermatozoa. It can be interpreted from these observations that cross-reacting antibodies developed against 33/30 kDa sperm proteins and 25, 33 kDa bacterial proteins in cows may affect the functional activity of spermatozoa leading to delayed fertility in heifers and cows. |
| تدمد: | 1439-0531 0936-6768 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9751a672f3d445b647a5268a9f471a2Test https://doi.org/10.1111/rda.13292Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d9751a672f3d445b647a5268a9f471a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390531 09366768 |
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