التفاصيل البيبلوغرافية
| العنوان: |
Identification and Characterization of Ribosomal Pausing Loci Responsible for Neural Development |
| المؤلفون: |
Martínez Brotons, Antonio |
| المساهمون: |
Yenush, Lynne Paula, Ikeuchi, Yoshiho, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia |
| المصدر: |
Repositorio Institucional de la Universitat Politècnica de València |
| بيانات النشر: |
Universitat Politècnica de València |
| سنة النشر: |
2017 |
| مصطلحات موضوعية: |
stat, psy |
| الوصف: |
[EN] Normal brain function requires correctly shaped and interconnected neurons. Abnormalities in brain development cause neurological disorders such as autism and epilepsy. The regulation of this process is dependent on the precise modulation of the expression of a variety of proteins. Thus, study of translation dynamics is key to the understanding of brain development and the creation of new therapies. In 2009 ‘ribosome profiling’ was introduced as new technique for the genome-wide analysis of protein translation. It allows the determination of ribosome occupancy along the transcriptome, which is directly related to translation efficiency. Thanks to the introduction of ribosome profiling an emerging mechanism of protein synthesis regulation, ribosomal pausing, has attracted increasing interest from researchers. The introduction of ribosome profiling has contributed to the interest of researchers in an emerging mechanism of protein synthesis regulation, ribosomal pausing. The causes of ribosomal pausing are not clear, but it can influence processes important for neuron differentiation such as mRNA transport across the cytoplasm. mTOR is a key regulator of translation in mammalian cells, having an important role in neuron morphogenesis. It can influence the elongation step of translation, and therefore could have an effect on ribosomal pausing. In this study, ribosome profiling data from mouse cortical neurons and embryonic stem cells is analyzed with the intention of discovering ribosomal pausing events that are potentially related to neural development. Samples treated with the mTOR inhibitor Torin 1 are included to assess the role of this important regulator in ribosomal pausing. The distribution of ribosomes along coding regions of the genome is studied, and the ribosomal pausing loci discovered are compared across the samples. Finally, some examples of translation pauses that could be important in neural development are discussed. Martínez Brotons, A. (2017). Identification and Characterization of Ribosomal ... |
| نوع الوثيقة: |
thesis |
| اللغة: |
English |
| العلاقة: |
http://hdl.handle.net/10251/88139Test |
| الإتاحة: |
http://hdl.handle.net/10251/88139Test |
| حقوق: |
other |
| رقم الانضمام: |
edsbas.BC2A5232 |
| قاعدة البيانات: |
BASE |
