التفاصيل البيبلوغرافية
| العنوان: |
Transient receptor potential vanilloid 1-mediated expression and secretion of endothelial cell-derived calcitonin gene-related peptide |
| المؤلفون: |
Luo, Dan dan962307@yahoo.com, Zhang, Yi-Wei1 eva_zyw1020@yahoo.com, Peng, Wei-Jie pengweijie@2118.cn, Peng, Jun1 junpeng@mail.csu.edu.cn, Chen, Qing-Quan1 bjcqq@163.com, Li, Dai1 lidaimail@yahoo.com.cn, Deng, Han-Wu1 deng_hw@sina.com, Li, Yuan-Jian yuan_jianli@yahoo.com |
| المصدر: |
Regulatory Peptides. Oct2008, Vol. 150 Issue 1-3, p66-72. 7p. |
| مصطلحات موضوعية: |
*CALCITONIN gene-related peptide, *NEUROTRANSMITTERS, *NERVES, *VASCULAR endothelium, *MESSENGER RNA, *POLYMERASE chain reaction, *LACTATE dehydrogenase |
| مستخلص: |
Abstract: Calcitonin gene-related peptide (CGRP), the principal transmitter in sensory nerves, could also be expressed in vascular endothelium. Transient receptor potential vanilloid 1(TRPV1), which modulates the synthesis and release of CGRP in sensory nerves, is also present in endothelial cells. The present study tested whether TRPV1 modulates the release and synthesis of CGRP in endothelial cells, and evaluated the protective effect of endothelial cell-derived CGRP. Human umbilical vein endothelial cells (HUVECs) were treated with capsaicin or hyperthermia. The level of CGRP mRNA was detected by RT-PCR, and protein level was measured by radioimmunoassay. Endothelial cell injury was induced by lysophosphatidylcholine, and evaluated by cell viability and lactate dehydrogenase activity. HUVECs expressed CGRP, both α- and β-subtype. Capsaicin increased the level of CGRP in the culture medium, and up-regulated the expression of CGRP in endothelial cells. Hyperthermia also increased the level of CGRP mRNA. These effects were abolished by capsazepine, a competitive antagonist of TRPV1. Capsaicin significantly attenuated the endothelial cell damage induced by LPC, which was prevented and aggravated by capsazepine or CGRP8–37, antagonist of CGRP receptor. These results indicate that TRPV1 also regulates the expression and secretion of endothelial cell-derived CGRP, which affords protective effects on endothelial cells. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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