التفاصيل البيبلوغرافية
العنوان:
Preliminary study for rapid determination of phycotoxins in microalgae whole cells using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
المؤلفون:
José M. Franco , Beatriz Paz , Pilar Riobó
المصدر:
Rapid Communications in Mass Spectrometry . 25:3627-3639
بيانات النشر:
Wiley, 2011.
سنة النشر:
2011
مصطلحات موضوعية:
Chromatography , Ciguatoxin , biology , Toxin , Organic Chemistry , Dinoflagellate , Domoic acid , Okadaic acid , medicine.disease_cause , biology.organism_classification , Mass spectrometry , Analytical Chemistry , chemistry.chemical_compound , chemistry , medicine , Sample preparation , Spectroscopy , Dinophysis
الوصف:
Rapid and sensitive methods for identification of several phycotoxins produced by microalgae species such as yessotoxins (YTXs) for Protoceratium reticulatum, okadaic acid (OA) and pectenotoxins (PTXs) for Prorocentrum spp. and Dinophysis spp., Palytoxins (PLTXs) for Ostreopsis spp., ciguatoxins (CTXs) for Gambierdiscus spp. or domoic acid (DA) for Pseudo-nitzschia spp. are of great importance to the shellfish and fish industry. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to detect several phycotoxins in whole cells of some microalgae which are known as toxin producers. To achieve an appropriate MALDI matrix and a sample preparation method, several matrices and solvent mixtures were tested. The most appropriate matrix system for toxin detection was obtained with 10 µg μL–1 of DHB in 0.1% TFA/ACN (3:7, v/v) by mixing the intact cells with the matrix solution directly on the MALDI target (dried-droplet technique). Toxin detection by this procedure is much faster than current procedures based on solvent extraction and chromatographic separation. This method allowed the rapid detection of main phycotoxins in some dinoflagellate cells of genus Ostreopsis, Prorocentrum, Protoceratium, Gambierdiscus, Dinophysis and diatoms from Pseudo-nitzschia genus. Copyright © 2011 John Wiley & Sons, Ltd.
تدمد:
0951-4198
الوصول الحر:
https://explore.openaire.eu/search/publication?articleId=doi_________::8312da5aca86f3727d04ca84ede03a34Test https://doi.org/10.1002/rcm.5264Test
حقوق:
OPEN
رقم الانضمام:
edsair.doi...........8312da5aca86f3727d04ca84ede03a34
قاعدة البيانات:
OpenAIRE
