دورية أكاديمية
Sphingosylphosphorylcholine induces alpha-smooth muscle actin expression in human lung fibroblasts and fibroblast-mediated gel contraction via S1P(2) receptor and Rho/Rho-kinase pathway
| العنوان: | Sphingosylphosphorylcholine induces alpha-smooth muscle actin expression in human lung fibroblasts and fibroblast-mediated gel contraction via S1P(2) receptor and Rho/Rho-kinase pathway |
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| المؤلفون: | Wang, X. Q., Mao, L. J., Fang, Q. H., Kobayashi, T., Kim, H. J., Sugiura, H., Kawasaki, S., Togo, S., Kamio, K., Liu, X., Rennard, S. I. |
| المساهمون: | Rennard, SI (reprint author), Univ Nebraska Med Ctr, 985910 Nebraska Med Ctr, Omaha, NE 68198 USA., Univ Nebraska Med Ctr, Dept Internal Med, Omaha, NE 68198 USA., Hebei United Univ, Affiliated Hosp, Dept Resp Dis, Tangshan, Hebei Province, Peoples R China., Peking Univ, Hosp 3, Res Ctr Occupat Med, Beijing 100191, Peoples R China., Capital Med Univ, Beijing Shijitan Hosp, Dept Pulm & Crit Care, Beijing 100038, Peoples R China., Mie Univ, Sch Med, Dept Pulm & Crit Care Med, Tsu, Mie 514, Japan., WonKuang Univ, Sch Med, SanBon Hosp, Dept Internal Med, Seoul, South Korea., Tohoku Univ, Grad Sch Med, Dept Resp Med, Aoba Ku, Sendai, Miyagi 9808574, Japan., Univ Tokyo, Grad Sch Med, Dept Resp Med, Tokyo, Japan., Juntendo Univ, Fac Med, Div Resp Med, Tokyo, Japan., Grad Sch Med, Tokyo, Japan., Nippon Med Sch, Dept Pulm Med Infect & Oncol, Tokyo 113, Japan., Univ Nebraska Med Ctr, 985910 Nebraska Med Ctr, Omaha, NE 68198 USA. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | prostaglandins other lipid mediators |
| سنة النشر: | 2014 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | SPC, Tissue repair, Fibroblast, SPHINGOSINE 1-PHOSPHATE, COLLAGEN MATRICES, RHO GTPASES, SPHINGOSINE-1-PHOSPHATE, MYOFIBROBLAST, CYTOSKELETON, INTEGRINS, MIGRATION, PROTEINS, GROWTH |
| الوصف: | Chronic airway diseases like COPD and asthma are usually accompanied with airway fibrosis. Myofibroblasts, which are characterized by expression of smooth muscle actin (alpha-SMA), play an important role in a variety of developmental and pathological processes, including fibrosis and wound healing. Sphingosylphosphorylcholine (SPC), a sphingolipid metabolite, has been implicated in many physiological and pathological conditions. The current study tested the hypothesis that SPC may modulate tissue remodeling by affecting the expression of a-SMA in human fetal lung fibroblast (HFL-1) and fibroblast mediated gel contraction. The results show that SPC stimulates a-SMA expression in HFL-1 and augments HFL-1 mediated collagen gel contraction in a time- and concentration-dependent manner. The a-SMA protein expression and fibroblast gel contraction induced by SPC was not blocked by TGF-beta 1 neutralizing antibody: However, it was significantly blocked by S1P2 receptor antagonist JTE-013, the Rho-specific inhibitor C3 exoenzyme, and a Rho-kinase inhibitor Y-27632. These findings suggest that SPC stimulates a-SMA protein expression and HFL-1 mediated collagen gel contraction via S1P2 receptor and Rho/Rho kinase pathway, and by which mechanism, SPC may be involved in lung tissue remodeling. (c) 2014 Published by Elsevier Inc. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336474600003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Test ; Biochemistry & Molecular Biology ; Cell Biology ; SCI(E) ; PubMed ; 2 ; ARTICLE ; srennard@unmc.edu ; 23-30 ; 108 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1098-8823 2212-196X |
| العلاقة: | PROSTAGLANDINS & OTHER LIPID MEDIATORS.2014,108,23-30.; 653384; http://hdl.handle.net/20.500.11897/189380Test; WOS:000336474600003 |
| DOI: | 10.1016/j.prostaglandins.2014.02.002 |
| الإتاحة: | https://doi.org/20.500.11897/189380Test https://doi.org/10.1016/j.prostaglandins.2014.02.002Test https://hdl.handle.net/20.500.11897/189380Test |
| رقم الانضمام: | edsbas.3B5BE142 |
| قاعدة البيانات: | BASE |
| تدمد: | 10988823 2212196X |
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| DOI: | 10.1016/j.prostaglandins.2014.02.002 |