دورية أكاديمية
Discovery and verification of urinary peptides in type 2 diabetes mellitus with kidney injury
| العنوان: | Discovery and verification of urinary peptides in type 2 diabetes mellitus with kidney injury |
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| المؤلفون: | Fu, Guangzhen, Du, Ye, Chu, Lina, Zhang, Man |
| المساهمون: | Zhang, M (reprint author), Peking Univ, Sch Clin Med 9, Dept Clin Lab, Beijing Shijitan Hosp, Beijing 100038, Peoples R China., Zhang, M (reprint author), Capital Med Univ, Beijing Shijitan Hosp, Dept Clin Lab, Beijing 100038, Peoples R China., Zhang, M (reprint author), Beijing Key Lab Urinary Cellular Mol Diagnost Bei, Beijing, Peoples R China., Zhang, M (reprint author), Chinese Med Doctor Assoc Lab Med, Beijing, Peoples R China., Peking Univ, Sch Clin Med 9, Dept Clin Lab, Beijing Shijitan Hosp, Beijing 100038, Peoples R China., Capital Med Univ, Beijing Shijitan Hosp, Dept Nephrol, Beijing 100038, Peoples R China., Capital Med Univ, Beijing Shijitan Hosp, Dept Clin Lab, Beijing 100038, Peoples R China., Beijing Key Lab Urinary Cellular Mol Diagnost Bei, Beijing, Peoples R China., Chinese Med Doctor Assoc Lab Med, Beijing, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | EXPERIMENTAL BIOLOGY AND MEDICINE |
| سنة النشر: | 2016 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | Urinary peptides, type 2 diabetes mellitus, diabetes nephropathy, magnetic bead processing, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, RENAL END-POINT, MICROALBUMINURIA EVER, CLINICAL-TRIAL, CAPILLARY-ELECTROPHORESIS, MASS-SPECTROMETRY, OXIDATIVE STRESS, NEPHROPATHY, PROTEINS, VITRONECTIN, BIOMARKERS |
| الوصف: | Varying degrees of renal injury could lead to different changes in urinary protein composition. We want to find urinary candidate peptide biomarkers in type 2 diabetic patients with different extents of kidney injury. Two sets of patients were recruited. Discovery set: weak cationic-exchange magnetic beads coupled with matrix-assisted laser desorption ionization time-of-flight mass spectrometry were used to profile the low-molecular weight peptidome in urine samples from type 2 diabetes patients with normoal-buminura and microalbuminuria. The differently expressed urinary peptides were screened by ClinProTools2.1 bioinformatics software and identified through nano-liquid chromatography-tandem mass spectrometry. Verification set: the above screened urinary peptides were validated by use matrix-assisted laser desorption ionization time-of-flight mass spectrometry on another group of type 2 diabetes patients with different extents use of kidney injury. In the screening and identification stages, seven urinary peptides were selected as the most promising biomarker candidates, and they were identified as fragments of vitronectin precursor, isoform 1 of fibrinogen alpha chain precursor, prothrombin precursor and inter-alpha-trypsin inhibitor heavy chain H4. The diagnostic efficacy of these urinary peptides was evaluated by area under the receiver operating characteristic curve, and they were 0.767, 0.768, 0.868, 0.910, 0.860, 0.843, and 0.865, respectively. In the verification stage, m/z 1743.9, 2154, 2175.5, and 2184.9 were decreased as albumin-to-creatinine (Alb/Cre) increased and m/z 2231.1, 2430.8, and 2756.1 were elevated as Alb/Cre rose. These small molecule peptides are related to type 2 diabetes kidney damage, and they may play an important role in monitoring type 2 diabetes. ; National High Technology Research and Development Program of China [2014AA020901]; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics [Z151100001615060] ; SCI(E) ; PubMed ; ARTICLE ; mzhang99@aliyun.com ; 11 ; 1186-1194 ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1535-3702 1535-3699 26846977 |
| العلاقة: | EXPERIMENTAL BIOLOGY AND MEDICINE.2016,241(11),1186-1194.; 1390980; http://hdl.handle.net/20.500.11897/435072Test; WOS:000378056900004 |
| DOI: | 10.1177/1535370216629007 |
| الإتاحة: | https://doi.org/20.500.11897/435072Test https://doi.org/10.1177/1535370216629007Test https://hdl.handle.net/20.500.11897/435072Test |
| رقم الانضمام: | edsbas.3D403DE2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15353702 15353699 26846977 |
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| DOI: | 10.1177/1535370216629007 |