Curcumin Reinforces MiR-29a Expression, Reducing Mesangial Fibrosis in a Model of Diabetic Fibrotic Kidney via Modulation of CB1R Signaling
| العنوان: | Curcumin Reinforces MiR-29a Expression, Reducing Mesangial Fibrosis in a Model of Diabetic Fibrotic Kidney via Modulation of CB1R Signaling |
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| المؤلفون: | Pey-Jium Chang, Lee-Wen Chen, Yung-Chien Hsu, Chia-Ching Liaw, Ya-Hsueh Shih, Shih-Jiun Lin, Chun-Liang Lin |
| المصدر: | Processes, Vol 9, Iss 694, p 694 (2021) Processes Volume 9 Issue 4 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, 030232 urology & nephrology, Bioengineering, urologic and male genital diseases, lcsh:Chemical technology, Diabetic nephropathy, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Type IV collagen, 0302 clinical medicine, Downregulation and upregulation, Fibrosis, medicine, Renal fibrosis, Chemical Engineering (miscellaneous), curcumin, lcsh:TP1-1185, Kidney, mesangial fibrosis, Process Chemistry and Technology, cannabinoid receptor type 1, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, chemistry, lcsh:QD1-999, Curcumin, Cancer research, Signal transduction, microRNA-29a |
| الوصف: | Renal fibrosis is a hallmark event in the pathogenesis of diabetic nephropathy. Considerable evidence now supports that multiple intracellular signaling pathways are critically involved in renal fibrosis. Previously, our studies have shown that dysregulation of the MicroRNA 29a (miR-29a)- or cannabinoid type 1 receptor (CB1R)-mediated signaling cascade in renal glomeruli substantially contributes to diabetic renal fibrosis. The purpose of the current study was to explore whether curcumin, a natural polyphenolic compound with potential renoprotective activity, could modulate the miR-29a/CB1R signaling axis to attenuate renal fibrosis. In this study, rat renal mesangial cells cultured in high glucose (HG) and the diabetic db/db mice were used as an in vitro and in vivo model of diabetes, respectively. Our results showed that in rat renal mesangial cells, curcumin treatment substantially counteracted HG-induced changes in the expressions of miR-29a, CB1R, peroxisome proliferator-activated receptor gamma (PPAR-γ), and a profibrotic marker type IV collagen (collagen IV), as assessed by quantitative Real-Time Polymerase chain reaction (RT-PCR). Furthermore, in the db/db mouse model, administration of curcumin markedly lowered urinary albumin excretion, and reduced deposition of extracellular matrices including collagen IV in renal tissues. Importantly, quantitative RT-PCR, in situ hybridization, and immunohistochemical analysis revealed that curcumin treatment consistently blocked diabetes-induced downregulation of miR-29a and upregulation of CB1R in renal glomeruli. Collectively, our study provides novel evidence showing that curcumin can rescue the dysregulated miR-29a/CB1R signaling pathway in glomerular mesangium to ameliorate diabetic renal fibrosis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2227-9717 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b813154542fafaedcdb1275007af845Test https://www.mdpi.com/2227-9717/9/4/694Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9b813154542fafaedcdb1275007af845 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22279717 |
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