التفاصيل البيبلوغرافية
| العنوان: |
FGFR1 SUMOylation coordinates endothelial angiogenic signaling in angiogenesis. |
| المؤلفون: |
Xiaolong Zhu, Cong Qiu, Yiran Wang, Yuanqing Jiang, Yefeng Chen, Linge Fan, Ruizhe Ren, Yunyun Wang, Yu Chen, Yanzhi Feng, Xiaofei Zhou, Yunhui Zhu, Zhen Ge, Dongwu Lai, Lingfeng Qin, Simons, Michael, Luyang Yu |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 6/28/2022, Vol. 119 Issue 26, p1-12, 33p |
| مصطلحات موضوعية: |
FIBROBLAST growth factor 2, NEOVASCULARIZATION, PROTEIN-tyrosine kinases |
| مستخلص: |
Angiogenesis contributes fundamentally to embryonic development, tissue homeostasis, and wound healing. Basic fibroblast growth factor (FGF2) is recognized as the first proangiogenic molecule discovered, and it facilitates angiogenesis by activating FGF receptor 1 (FGFR1) signaling in endothelial cells. However, the precise roles of FGFR and the FGF/FGFR signaling axis in angiogenesis remain unclear, especially because of the contradictory phenotypes of in vivo FGF and FGFR gene deficiency models. Our previous study results suggested a potential role of posttranslational small ubiquitin-like modifier modification (SUMOylation), with highly dynamic regulatory features, in vascular development and disorder. Here, we identified SENP1-regulated endothelial FGFR1 SUMOylation at conserved lysines responding to proangiogenic stimuli, while SENP1 functioned as the deSUMOylase. Hypoxia-enhanced FGFR1 SUMOylation restricted the tyrosine kinase activation of FGFR1 by modulating the dimerization of FGFR1 and FGFR1 binding with its phosphatase PTPRG. Consequently, it facilitated the recruitment of FRS2a to VEGFR2 but limited additional recruitment of FRS2a to FGFR1, supporting the activation of VEGFA/VEGFR2 signaling in endothelial cells. Furthermore, SUMOylation-defective mutation of FGFR1 resulted in exaggerated FGF2/FGFR1 signaling but suppressed VEGFA/VEGFR2 signaling and the angiogenic capabilities of endothelial cells, which were rescued by FRS2a overexpression. Reduced angiogenesis and endothelial sprouting in mice bearing an endothelial-specific, FGFR1 SUMOylation-defective mutant confirmed the functional significance of endothelial FGFR1 SUMOylation in vivo. Our findings identify the reversible SUMOylation of FGFR1 as an intrinsic fine-tuned mechanism in coordinating endothelial angiogenic signaling during neovascularization; SENP1-regulated FGFR1 SUMOylation and deSUMOylation controls the competitive recruitment of FRS2a by FGFR1 and VEGFR2 to switch receptor-complex formation responding to hypoxia and normoxia angiogenic environments. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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