التفاصيل البيبلوغرافية
| العنوان: |
Modifications in the T arm of tRNA globally determine tRNA maturation, function, and cellular fitness. |
| المؤلفون: |
Schultz, Sarah K., Katanski, Christopher D., Halucha, Mateusz, Peña, Noah, Fahlman, Richard P., Tao Pan, Kothe, Ute |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 6/25/2024, Vol. 121 Issue 26, p1-9, 50p |
| مستخلص: |
Almost all elongator tRNAs (Transfer RNAs) harbor 5-methyluridine 54 and pseudouridine 55 in the T arm, generated by the enzymes TrmA and TruB, respectively, in Escherichia coli. TrmA and TruB both act as tRNA chaperones, and strains lacking trmA or truB are outcompeted by wild type. Here, we investigate how TrmA and TruB contribute to cellular fitness. Deletion of trmA and truB in E. coli causes a global decrease in aminoacylation and alters other tRNA modifications such as acp³U47. While overall protein synthesis is not affected in ΔtrmA and ΔtruB strains, the translation of a subset of codons is significantly impaired. As a consequence, we observe translationally reduced expression of many specific proteins, that are either encoded with a high frequency of these codons or that are large proteins. The resulting proteome changes are not related to a specific growth phenotype, but overall cellular fitness is impaired upon deleting trmA and truB in accordance with a general protein synthesis impact. In conclusion, we demonstrate that universal modifications of the tRNA T arm are critical for global tRNA function by enhancing tRNA maturation, tRNA aminoacylation, and translation, thereby improving cellular fitness irrespective of the growth conditions which explains the conservation of trmA and truB. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
