Imaging individual neurons in the retinal ganglion cell layer of the living eye
| العنوان: | Imaging individual neurons in the retinal ganglion cell layer of the living eye |
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| المؤلفون: | Mina M. Chung, Koji Nozato, Sarah Walters, David R. Williams, Christina Schwarz, Robin Sharma, Kenichi Saito, Lisa R. Latchney, Jie Zhang, William S. Fischer, Qiang Yang, Jennifer J. Hunter, Charles E. Granger, Ethan A. Rossi, Tomoaki Kawakami |
| المصدر: | Proceedings of the National Academy of Sciences. 114:586-591 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Retinal Ganglion Cells, Optical Phenomena, genetic structures, Giant retinal ganglion cells, Biology, 01 natural sciences, Retinal ganglion, 010309 optics, Ophthalmoscopy, Macular Degeneration, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Species Specificity, 0103 physical sciences, medicine, Animals, Humans, Retina, Multidisciplinary, medicine.diagnostic_test, Intrinsically photosensitive retinal ganglion cells, Glaucoma, Retinal, Anatomy, Biological Sciences, Macular degeneration, medicine.disease, Macaca mulatta, eye diseases, Macaca fascicularis, medicine.anatomical_structure, chemistry, Retinal ganglion cell, Retinal Cone Photoreceptor Cells, 030221 ophthalmology & optometry, Female, sense organs, Neuroscience |
| الوصف: | Although imaging of the living retina with adaptive optics scanning light ophthalmoscopy (AOSLO) provides microscopic access to individual cells, such as photoreceptors, retinal pigment epithelial cells, and blood cells in the retinal vasculature, other important cell classes, such as retinal ganglion cells, have proven much more challenging to image. The near transparency of inner retinal cells is advantageous for vision, as light must pass through them to reach the photoreceptors, but it has prevented them from being directly imaged in vivo. Here we show that the individual somas of neurons within the retinal ganglion cell (RGC) layer can be imaged with a modification of confocal AOSLO, in both monkeys and humans. Human images of RGC layer neurons did not match the quality of monkey images for several reasons, including safety concerns that limited the light levels permissible for human imaging. We also show that the same technique applied to the photoreceptor layer can resolve ambiguity about cone survival in age-related macular degeneration. The capability to noninvasively image RGC layer neurons in the living eye may one day allow for a better understanding of diseases, such as glaucoma, and accelerate the development of therapeutic strategies that aim to protect these cells. This method may also prove useful for imaging other structures, such as neurons in the brain. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a2903af34fa6889fa60d9affcffc18cTest https://doi.org/10.1073/pnas.1613445114Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0a2903af34fa6889fa60d9affcffc18c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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