Constitutively active UVR8 photoreceptor variant in Arabidopsis
| العنوان: | Constitutively active UVR8 photoreceptor variant in Arabidopsis |
|---|---|
| المؤلفون: | Kris Gevaert, Ruohe Yin, Melanie Binkert, Geert De Jaeger, Marc Heijde, Florence Ares-Orpel, Geert Persiau, Eveline Van De Slijke, Luca Rizzini, Jonah Nolf, Roman Ulm |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences, Vol. 110, No 50 (2013) pp. 20326-20331 Proceedings of the National Academy of Sciences |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 0106 biological sciences, UVR8, Chromosomal Proteins, Non-Histone, Arabidopsis, Cell Cycle Proteins, 01 natural sciences, Anthocyanins, chemistry.chemical_compound, Tandem Mass Spectrometry, Aromatic amino acids, Arabidopsis Proteins/genetics/metabolism, Alanine, 0303 health sciences, Multidisciplinary, biology, Phytochrome, Photoreceptors, Plant/genetics, food and beverages, Biological Sciences, Plants, Genetically Modified, Cell biology, ddc:580, Phenotype, Mutation, Missense/genetics, Anthocyanins/metabolism, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Photomorphogenesis, Genetic Engineering, Photoreceptors, Plant, Ubiquitin-Protein Ligases, Mutation, Missense, Repressor, Real-Time Polymerase Chain Reaction, Chromosomal Proteins, Non-Histone/genetics, 03 medical and health sciences, Ubiquitin-Protein Ligases/metabolism, Two-Hybrid System Techniques, Immunoprecipitation, Arabidopsis/genetics, 030304 developmental biology, Cell Cycle Proteins/metabolism, Arabidopsis Proteins, fungi, Tryptophan, biology.organism_classification, Molecular biology, eye diseases, chemistry, sense organs, Chromatography, Liquid, 010606 plant biology & botany |
| الوصف: | Arabidopsis thaliana UV RESISTANCE LOCUS 8 (UVR8) is a UV-B photoreceptor that initiates photomorphogenic responses underlying acclimation and UV-B tolerance in plants. UVR8 is a homodimer in its ground state, and UV-B exposure results in its instantaneous monomerization followed by interaction with CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), a major factor in UV-B signaling. UV-B photoreception by UVR8 is based on intrinsic tryptophan aromatic amino acid residues, with tryptophan-285 as the main chromophore. We generated transgenic plants expressing UVR8 with a single amino acid change of tryptophan-285 to alanine. UVR8(W285A) appears monomeric and shows UV-B-independent interaction with COP1. Phenotypically, the plants expressing UVR8(W285A) exhibit constitutive photomorphogenesis associated with constitutive activation of target genes, elevated levels of anthocyanins, and enhanced, acclimation-independent UV-B tolerance. Moreover, we have identified COP1, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 and 2 (RUP1 and RUP2), and the SUPPRESSOR OF PHYA-105 (SPA) family as proteins copurifying with UVR8(W285A). Whereas COP1, RUP1, and RUP2 are known to directly interact with UVR8, we show that SPA1 interacts with UVR8 indirectly through COP1. We conclude that UVR8(W285A) is a constitutively active UVR8 photoreceptor variant in Arabidopsis, as is consistent with the crucial importance of monomer formation and COP1 binding for UVR8 activity. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfcf5c174669cbb8e2c1a113fb35a5b0Test https://doi.org/10.1073/pnas.1314336110Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bfcf5c174669cbb8e2c1a113fb35a5b0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
|---|