دورية
In vitro and genomic mining studies of anti-Clostridium perfringensCompounds Derived from Bacillus amyloliquefaciens
| العنوان: | In vitro and genomic mining studies of anti-Clostridium perfringensCompounds Derived from Bacillus amyloliquefaciens |
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| المؤلفون: | Li, Charles, Yan, Xianghe, Yang, Yishan, Nou, Xiangwu, Sun, Zhifeng, Lillehoj, Hyun S., Lu, Mingling, Harlow, KaLynn, Rivera, Israel |
| المصدر: | Poultry Science; 20240101, Issue: Preprints |
| مستخلص: | Clostridium perfringensis an important opportunistic microorganism in commercial poultry production that is implicated in necrotic enteritis (NE) outbreaks. This disease poses a severe financial burden on the global poultry industry, causing estimated annual losses of $6 billion globally. The ban on in-feed antibiotic growth promoters has spurred investigations into approaches of alternatives to antibiotics, among which Bacillusprobiotics have demonstrated varying degrees of effectiveness against NE. However, the precise mechanisms underlying Bacillus-mediated beneficial effects on host responses in NE remain to be further elucidated. In this manuscript, we conducted in vitro and genomic mining analysis to investigate anti-C. perfringensactivity observed in the supernatants derived from 2 Bacillus amyloliquefaciensstrains (FS1092 and BaD747). Both strains demonstrated potent anti-C. perfringensactivities in in vitrostudies. An analysis of genomes from 15 B. amyloliquefaciens, 11 B. velezensis,and 2 B. subtilisstrains has revealed an intriguing clustering pattern among strains known to possess anti-C. perfringensactivities. Furthermore, our investigation has identified 7 potential antimicrobial compounds, predicted as secondary metabolites through antiSMASH genomic mining within the published genomes of B. amyloliquefaciensspecies. Based on in vitro analysis, BaD747 may have the potential as a probiotic in the control of NE. These findings not only enhance our understanding of B. amyloliquefaciens's action against C. perfringensbut also provide a scientific rationale for the development of novel antimicrobial therapeutic agents against NE. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00325791 15253171 |
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| DOI: | 10.1016/j.psj.2024.103871 |