المؤلفون: Michael H. Kogut, Yuhua Z. Farnell, Chuan-Yu Hsu, Dan Zhao, Kenneth J. Genovese, Jason T. Lee

المصدر: Poultry Science, Vol 99, Iss 1, Pp 11-20 (2020)
Poultry Science

مصطلحات موضوعية: Monocarboxylic Acid Transporters, medicine.medical_specialty, Muscle Proteins, Pectoralis Muscles, Avian Proteins, 03 medical and health sciences, chemistry.chemical_compound, Downregulation and upregulation, Internal medicine, Lactate dehydrogenase, medicine, Animals, Lactic Acid, monocarboxylate transporters, Poultry Diseases, 030304 developmental biology, lcsh:SF1-1100, Monocarboxylate transporter, 0303 health sciences, Messenger RNA, biology, L-Lactate Dehydrogenase, 0402 animal and dairy science, Broiler, Transporter, lactate dehydrogenase, 04 agricultural and veterinary sciences, General Medicine, Immunology, Health and Disease, 040201 dairy & animal science, Enzyme assay, wooden breast, Endocrinology, chemistry, broiler lactate metabolism, biology.protein, Animal Science and Zoology, lcsh:Animal culture, LDH isoenzyme activity, Chickens, Homeostasis

الوصف: Wooden breast (WB) results in significant losses to the broiler industry due to reductions in meat quality. While the etiology of WB is unknown, it is believed to be associated with localized hypoxia and decreased lactate levels in skeletal muscles, indicating the presence of altered lactate metabolism in WB. We hypothesized that the expression levels of the major signaling molecules that control lactate metabolism, including lactate dehydrogenases (LDHA and LDHB) and monocarboxylate transporters (MCT1 and MCT4), were altered in WB. Therefore, the objectives of this study were to evaluate whether there were changes in mRNA and protein levels of LDHA, LDHB, MCT1, and MCT4 in WB compared to normal breast (NB) muscles. Biochemical analysis for LDH enzyme activity in NB and WB muscles was studied. MicroRNA375 (miR-375) expression, known to be inversely associated with LDHB protein expression in human cells, was also investigated. The level of LDHA mRNA was 1.7-fold lower in WB tissues than in NB tissues (P < 0.0001). However, the LDHA protein levels were similar in WB and NB tissues. In contrast, the levels of LDHB mRNA and protein were 8.4-fold higher (P < 0.002) and 13.6-fold higher (P < 0.02) in WB than in NB tissues, respectively. The level of miR-375 was not different between WB and NB muscles. The specific LDH isoenzyme activity that converted lactate to pyruvate was 1.8-fold lower in WB compared to NB tissues (P < 0.01). The level of MCT1 mRNA was 2.3-fold higher in WB than those in NB muscles (P < 0.02). However, this upregulation was not observed with MCT1 protein expression levels. The expression levels of MCT4 mRNA and protein were elevated 2.8-fold (P < 0.02) and 3.5-fold (P < 0.004) in WB compared to NB tissues, respectively. Our current findings suggest the potential roles of LDHB and MCT4 on lactate metabolism and provide a unique molecular elucidation for altered lactate homeostasis in WB muscles of broilers.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7a0e6c61b1dc8570c622de9138d6f55Test
http://www.sciencedirect.com/science/article/pii/S0032579119578440Test

المؤلفون: Jiaolong Li, Zuodong Chen, Tong Xing, Yun Jiang, Feng Gao, Lin Zhang

المصدر: Poultry Science, Vol 100, Iss 2, Pp 918-925 (2021)
Poultry Science

مصطلحات موضوعية: Nrf2 signaling pathway, medicine.medical_specialty, medicine.medical_treatment, Intraperitoneal injection, hydrogen peroxide, antioxidant capacity, medicine.disease_cause, broiler, Antioxidants, Pectoralis Muscles, Metabolism and Nutrition, Random Allocation, 03 medical and health sciences, chemistry.chemical_compound, Internal medicine, Lactate dehydrogenase, medicine, Animals, 030304 developmental biology, lcsh:SF1-1100, chemistry.chemical_classification, reactive oxygen species, 0303 health sciences, Chemistry, Glutathione peroxidase, 0402 animal and dairy science, Broiler, 04 agricultural and veterinary sciences, General Medicine, Metabolism, Oxidants, Malondialdehyde, 040201 dairy & animal science, Oxidative Stress, Endocrinology, Dietary Supplements, glycolysis metabolism, Animal Science and Zoology, lcsh:Animal culture, Chickens, Oxidation-Reduction, Anaerobic exercise, Oxidative stress

الوصف: Oxidative stress has always been a hot topic in poultry science. However, studies concerning the effects of redox status and glucose metabolism induced by hydrogen peroxide (H2O2) in the breast muscle of broilers have been rarely reported. This study was aimed to evaluate the impact of intraperitoneal injection of H2O2 on oxidative damage and glycolysis metabolism of breast muscle in broilers. We also explored the activation of the nuclear factor erythroid 2–related factor 2 (Nrf2) signaling pathway to provide possible mechanism of the redox imbalance. Briefly, a total of 320 one-day-old Arbor Acres chicks were randomly divided into 5 treatments with 8 replicates of 8 birds each (noninjected control, 0.75% saline-injected, 2.5, 5.0, and 10.0% H2O2-injected treatments). Saline group was intraperitoneally injected with physiological saline (0.75%) and H2O2 groups received an intraperitoneal injection of H2O2. The dosage of the injection was 1.0 mL/kg BW. All birds in the saline and H2O2 groups were injected on days 16 and 37 of the experimental period. At 42 d of age, 40 birds (8 cages per group and one chicken per cage) were selected to be stunned electrically (50 V, alternating current, 400 Hz for 5 s each one), and then immediately slaughtered via exsanguination. The results showed that broilers in the H2O2 injection group linearly exhibited higher contents of reactive oxygen species, carbonyl and malondialdehyde, and lower total antioxidant capacity and glutathione peroxidase activities. With the content of H2O2 increased, the H2O2 groups linearly downregulated the mRNA expressions of GPX, CAT, HMOX1, NQO1, and Nrf2 and its downstream target genes. In addition, H2O2 increased serum activities of creatine kinase and lactate dehydrogenase. Meanwhile, in the pectoral muscle, the glycogen content was linearly decreased, and the lactate content was linearly increased in muscle of broilers injected with H2O2. In addition, the activities of glycolytic enzymes including pyruvate kinase, hexokinase, and lactate dehydrogenase were linearly increased after exposure to H2O2. In conclusion, H2O2 injection could impair antioxidant status and enhance anaerobic metabolism of breast muscle in broilers.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16ff3fe060e7bceabda78b0cd302ef74Test
http://www.sciencedirect.com/science/article/pii/S0032579120308816Test

المؤلفون: N. Afzali, Pouyan Malekinezhad, Abbas Mohammadi, Arash Omidi, Seyed Homayoun Farhangfar, Laura E. Ellestad

المصدر: Poultry Science
Poultry Science, Vol 100, Iss 2, Pp 797-809 (2021)

مصطلحات موضوعية: Ochratoxin A, Male, Aflatoxin, Aflatoxin B1, Berberine, ochratoxicosis, broiler, Feed conversion ratio, Metabolism and Nutrition, 03 medical and health sciences, chemistry.chemical_compound, Random Allocation, Animal science, Lactate dehydrogenase, liver lesion, Animals, aflatoxicosis, 030304 developmental biology, lcsh:SF1-1100, chemistry.chemical_classification, 0303 health sciences, Glutathione peroxidase, 0402 animal and dairy science, Broiler, 04 agricultural and veterinary sciences, General Medicine, 040201 dairy & animal science, Animal Feed, Ochratoxins, Diet, chemistry, Urea, Animal Science and Zoology, Liver function, lcsh:Animal culture, Chickens

الوصف: Many types of mycotoxins are found in food sources contaminated with fungi, and if these are ingested in large quantities or over a long period, they can affect the health of humans and domestic animals. Berberine (BBR) is a plant alkaloid with multiple pharmacological functions. This study aimed to investigate the effect of different levels of the plant alkaloid BBR on reducing toxic effects of aflatoxin B1 (AFB) and ochratoxin A (OTA) in broilers by examining performance characteristics, blood biochemistry, antioxidant systems, ileum morphology, and histopathology of the liver. The experiment was performed with 288 Ross 308 broilers reared in floor pens for 42 d in a randomized design with 9 treatments. Each treatment was replicated 4 times, and each replicate contained 8 chicks. Experimental treatments included (1) negative control diet with no additives (NC); (2) NC + 2 ppm AFB (positive control AFB; PCAFB); (3) NC + 2 ppm OTA (positive control OTA; PCOTA); (4) PCAFB + 200 mg/kg BBR; (5) PCAFB + 400 mg/kg BBR; (6) PCAFB + 600 mg/kg BBR; (7) PCOTA + 200 mg/kg BBR; (8) PCOTA + 400 mg/kg BBR; and (9) PCOTA + 600 mg/kg BBR. Compared with NC, feeding PCAFB and PCOTA diets reduced average daily feed intake, weight gain, serum concentrations of superoxide dismutase, glutathione peroxidase, and the length and width of ileum villi (P < 0.05). At the same time, these parameters increased in birds fed PCAFB or PCOTA diets supplemented with 600 mg/kg of BBR (P < 0.05). Feeding PCAFB and PCOTA diets increased feed conversion ratio (FCR), serum aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT) activities, serum urea, and liver lesions compared with NC. By contrast, compared with PCAFB and PCOTA, adding 600 mg/kg BBR decreased FCR, AST, LDH, ALT, and GGT activities, urea, and liver lesions (P < 0.05). Overall, supplementation with 600 mg/kg BBR may improve growth performance, liver function, and antioxidant status of broilers fed diets contaminated with AFB and OTA.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::491c1c250369f67c492acace712cf8e2Test
http://europepmc.org/articles/PMC7858088Test

المؤلفون: Shengxin Fan, Yuanfang Li, Guirong Sun, Wenting Li, Guoxi Li, Pengtao Yuan, Xiaojun Liu, Wenjiao Jin, Xiangtao Kang, Bin Zhai, Yadong Tian

المصدر: Poultry Science
Poultry Science, Vol 101, Iss 2, Pp 101557-(2022)

مصطلحات موضوعية: Muscle tissue, Striated muscle tissue, Lactate dehydrogenase A, Biology, SF1-1100, Myoblasts, miR-29b-1-5p, medicine, Myocyte, Animals, education, Transcription factor, GENETICS AND MOLECULAR BIOLOGY, Cell Proliferation, education.field_of_study, ANKRD9, Cadherin, Skeletal muscle, Cell Differentiation, General Medicine, Cell cycle, Cell biology, Animal culture, MicroRNAs, medicine.anatomical_structure, chicken primary myoblast, Animal Science and Zoology, muscle development, Chickens

الوصف: Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts (CPMs); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 (ANKRD9), lactate dehydrogenase A (LDHA), transcription factor 12 (TCF12), FAT atypical cadherin 1 (FAT1), lin-9 homolog (LIN9), and integrin beta 3 binding protein (ITGB3BP), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9. Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9. This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64715bde0b57d9a803001f97d29c9f71Test
http://europepmc.org/articles/PMC8639469Test

المؤلفون: Jiangshui Wang, Chianning Heng, Dianchun Wang, Lei Xia, Yuanyuan Wang, Tian-Yu Guo, Kaixuan Li, Lei Jiang, Xiuan Zhan

المصدر: Poultry Science, Vol 99, Iss 4, Pp 2017-2025 (2020)
Poultry Science

مصطلحات موضوعية: Male, Hot Temperature, medicine.medical_treatment, Oxidative phosphorylation, Adrenocorticotropic hormone, oxidative damage, Management and Production, Biology, Heat Stress Disorders, heat stress, Random Allocation, 03 medical and health sciences, chemistry.chemical_compound, animal comfort, Animal science, Lactate dehydrogenase, medicine, Animals, 030304 developmental biology, lcsh:SF1-1100, 0303 health sciences, yellow-feathered broiler, Growth factor, 0402 animal and dairy science, 04 agricultural and veterinary sciences, General Medicine, 040201 dairy & animal science, metabolomics, Hormones, Hsp70, Heat stress, Cold Temperature, Oxidative Stress, chemistry, Metabolome, biology.protein, Female, Animal Science and Zoology, Creatine kinase, lcsh:Animal culture, Chickens, Heat-Shock Response, Hormone

الوصف: There is no information regarding the influence of heat stress (HS) on host metabolic profile. In this study, we investigated the effects of different environmental temperatures on oxidative status, hormone levels, HS indicators, and plasma metabolites in broilers. A total of 1,680 yellow-feather broilers (28 D old) were randomly allotted to 4 groups with 6 replicates. The broilers (29–57 D old) were maintained in thermostatic rooms (20°C, 25°C, 28°C, and 30°C) for 28 consecutive days. The results showed that the plasma cortisol and adrenocorticotropic hormone levels and creatine kinase and lactate dehydrogenase activities gradually increased when the temperature increased from 20°C to 30°C. However, the insulin-like growth factor-І level decreased gradually. Furthermore, heat shock protein 70 expression significantly increased in the liver and breast muscle (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adfe3020660634f329332e804721bceaTest
http://www.sciencedirect.com/science/article/pii/S0032579119580488Test

المؤلفون: Rajendar P. Gupta, Preeti Bagri, Mamta Kumari, Deepika Lather

المصدر: Poultry Science, Vol 99, Iss 4, Pp 1875-1887 (2020)
Poultry Science

مصطلحات موضوعية: chicken, Glutathione reductase, Aspartate transaminase, Lymphocyte proliferation, Withania, Biology, Withania somnifera, Plant Roots, immune response, lesions, Superoxide dismutase, Andrology, Random Allocation, 03 medical and health sciences, chemistry.chemical_compound, Anti-Infective Agents, Lactate dehydrogenase, Escherichia coli, Animals, Escherichia coli Infections, Poultry Diseases, 030304 developmental biology, lcsh:SF1-1100, 0303 health sciences, Dose-Response Relationship, Drug, Plant Extracts, 0402 animal and dairy science, Broiler, 04 agricultural and veterinary sciences, General Medicine, Immunology, Health and Disease, biology.organism_classification, Animal Feed, 040201 dairy & animal science, Diet, Alanine transaminase, chemistry, Dietary Supplements, biology.protein, Animal Science and Zoology, lcsh:Animal culture, Chickens

الوصف: The present study was undertaken to investigate the effect of aqueous Withania somnifera root (WSR) extract in broiler chicks experimentally infected with Escherichia coli O78 @ 107 CFU/0.5 ml intraperitoneally. Clinical signs and mortality due to colibacillosis observed in infected chicks were mild and lasted for short duration in WSR extract supplemented group as compared with the nonsupplemented group. A significant increase in serum alanine transaminase, aspartate transaminase, lactate dehydrogenase, and creatine phosphokinase activities and a decrease in total protein and albumin concentrations were observed in the infected groups, though these changes were of lower magnitude in WSR extract supplemented group. A significantly higher activity of oxidative blood parameters such as superoxide dismutase, catalase, glutathione reductase, and glutathione-S-transferase enzymes were noticed in WSR extract supplemented group. The WSR extract supplemented group revealed significantly higher E. coli–specific antibody titer and enhanced lymphocyte proliferation response as compared with the nonsupplemented group. The gross and histopathological lesions of colibacillosis were mild in the WSR extract–supplemented infected group as compared with the nonsupplemented infected group. Withania somnifera root extract supplementation produced 31.48 and 34.38% protection in the gross and histopathological lesions in E. coli infected chicks, respectively. It is concluded that supplementation of 20% WSR extract @ 20 ml/L of water caused a reduction in the severity, mortality, and recovery period of E. coli infection and enhanced the humoral and cellular immune responses suggesting its protective effect on limiting the pathology of E. coli infection in broiler chickens.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1956d48e3ad2f1cc76477bd80339dc7dTest
http://www.sciencedirect.com/science/article/pii/S0032579119578191Test

المؤلفون: G. Attia, Fayiz M. Reda, T. K. El-Rayes, Mahmoud Alagawany, Mahmoud E. Farahat, Mohamed T. El-Saadony

المصدر: Poultry Science, Vol 100, Iss 8, Pp 101266-(2021)
Poultry Science

مصطلحات موضوعية: intestinal microbiota, MANAGEMENT AND PRODUCTION, Coturnix, Quail, Feed conversion ratio, SF1-1100, 03 medical and health sciences, chemistry.chemical_compound, Animal science, licorice, blood, biology.animal, Lactate dehydrogenase, quails, Escherichia coli, Glycyrrhiza, medicine, Animals, Gizzard, 030304 developmental biology, 0303 health sciences, medicine.diagnostic_test, biology, Triglyceride, 0402 animal and dairy science, glob (programming), 04 agricultural and veterinary sciences, General Medicine, biology.organism_classification, Animal Feed, 040201 dairy & animal science, Diet, Gastrointestinal Microbiome, Animal culture, chemistry, Dietary Supplements, Animal Science and Zoology, Lipid profile, Chickens, performance

الوصف: This study aimed to assess the impacts of licorice (Glycyrrhiza glabra) on the growth performance, carcass traits, intestinal microbiota, liver and kidney functions, immunity, oxidative status, and lipid profile of Japanese quails. A total of 200 one-week-old unsexed Japanese quails with an average initial body weight of 26.24 ± 0.2 g were randomly distributed into 5 equal groups of 40 birds and further subdivided into 5 replicates. The first (control) group was fed a diet without any licorice, while licorice powder was added at levels of 250, 500, 750, and 1000 mg per kg diet in the second, third, fourth, and fifth groups, respectively. At the age of 3 wk, the group of quail fed on a diet supplemented with 750 and 1000 mg licorice/kg of diet gained the highest body weight (BW) and daily body weight (DBW), while attaining the lowest feed conversion ratio (FCR) compared to other groups. Meanwhile, groups fed diets with licorice at levels of 0 and 250 mg/kg showed the highest feed intake. After the 5-wk feeding trial, the highest BW and DBW values, and the lowest FCR were recorded in the group fed with 750 mg licorice/kg diet. The different treatments produced no significant differences (P > 0.05) in quail carcass characteristics, including percentages of carcass, liver, gizzard, heart, giblets, and dressing. The blood of the group fed a 750 mg licorice diet had higher contents of total protein and GLOB, while its contents of A/G%, lactate dehydrogenase (LDH), total cholesterol, triglyceride (TG), and low density lipoprotein (LDL) were lower. Apart from the high level of licorice (1000 mg/kg), the MDA level was linearly and quadratically (P = 0.0413 and 0.001) decreased with different licorice groups, while superoxide dismutase (SOD), total antioxidant capacity (TAC), immunoglobulin G (IgG), and M (IgM) were quadratically increased when compared to the control group. Licorice supplementation resulted in marked reductions in the number of total bacteria, coliforms, E. coli, and Salmonella, compared to those in the control. In conclusion, the inclusion of licorice at levels of 750 and 1000 mg/kg into the diet of Japanese quail enhances the animal's performance, immunity, antioxidant capacity, and maintains a healthy gut microbiota.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42c08a4ec11ebab30bc4334da3f2ebf2Test
http://www.sciencedirect.com/science/article/pii/S003257912100300XTest

المؤلفون: Elke Albrecht, C. Miersch, R. Zitnan, Claudia Kalbe, Mikuláš Levkut, Monika Röntgen, Viera Revajová

المصدر: Poultry Science

مصطلحات موضوعية: Enterococcus faecium, broiler, law.invention, Random Allocation, 03 medical and health sciences, chemistry.chemical_compound, Probiotic, Animal science, Salmonella, law, Lactate dehydrogenase, Animals, Myocyte, Muscle, Skeletal, Poultry Diseases, 030304 developmental biology, Salmonella Infections, Animal, 0303 health sciences, biology, Probiotics, 0402 animal and dairy science, Broiler, 04 agricultural and veterinary sciences, General Medicine, Immunology, Health and Disease, biology.organism_classification, Animal Feed, 040201 dairy & animal science, Diet, Isocitrate dehydrogenase, Salmonella enteritidis, chemistry, Enterococcus, biology.protein, Muscle, Animal Science and Zoology, Creatine kinase, Chickens, probiotic

الوصف: The present study was conducted to assess the effects of the probiotic Enterococcus faecium AL41 (EF) and of the enteric pathogen Salmonella Enteritidis PT4 (SE) on the development of posthatch pectoralis major muscle (PM) of broiler chicks. The four experimental groups were control (CON), EF, SE, and EF+SE (EFSE). EF and SE were given per os from days 1 to 7 and at day 4 posthatch, respectively. Muscle samples from 6 chicks per group were taken at day 8 (D8) and day 11 (D11) to evaluate PM myofiber growth, capillarization, DNA, RNA, and protein content, as well as enzyme activities (isocitrate dehydrogenase, lactate dehydrogenase, creatine kinase). PM growth rate was 7.45 ± 2.7 g/d in non-SE groups (CON, EF) and 5.10 ± 1.82 g/d in SE-infected groups (P < 0.02). Compared with group CON, application of bacteria (groups EF and SE) reduced the fiber cross-sectional area (246 and 262 vs. 347 ± 19 μm2) and the number of myonuclei per fiber (0.66 and 0.64 vs. 0.79 ± 0.03). At D11, hypertrophic myofiber growth normalized in the EF group, but negative effects persisted in SE and EFSE birds contributing to lower daily PM gain. In addition, SE infection strongly disturbed PM capillarization. Negative effects on capillary cross-sectional area and on the area (%) covered by capillaries persisted until D11 in the SE group, whereas pre-feeding of EF restored capillarization in the EFSE group to control levels. We conclude that supplementation of the probiotic bacteria EF AL41 had positive effects on PM capillarization and, thus, on delivery of O2, supply of nutrients, and removal of metabolites. Supplementation of probiotic bacteria might therefore reduce energetic stress and improve muscle health and meat quality during SE infection.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5998fec6195b764f1ae6e925e653dea4Test
https://doi.org/10.3382/ps/pey561Test

المؤلفون: Chunmei Li, Guofeng Han, Dan Shen, Pengyuan Dai, Mengxue Xi

المصدر: Poultry Science
Poultry Science, Vol 101, Iss 2, Pp 101593-(2022)

مصطلحات موضوعية: Antioxidant, medicine.medical_treatment, Necroptosis, Cell, necroptosis, Chick Embryo, SF1-1100, chicken alveolar epithelial cell, chemistry.chemical_compound, Lactate dehydrogenase, NLR Family, Pyrin Domain-Containing 3 Protein, medicine, Animals, Propidium iodide, Viability assay, GENETICS AND MOLECULAR BIOLOGY, pyroptosis, Pyroptosis, General Medicine, Animal culture, Hydroquinones, Cell biology, medicine.anatomical_structure, fine particulate matter, chemistry, Alveolar Epithelial Cells, TBHQ, Particulate Matter, Animal Science and Zoology, Chickens, Intracellular

الوصف: Fine particulate matter (PM2.5) from poultry houses has adverse effects on the health of animals and workers. Tert-butylhydroquinone (TBHQ), an antioxidant, is widely used in feed additives. The present study investigated the effects of TBHQ on broiler house PM2.5-induced damage in chicken primary alveolar epithelial cells (AECII) extracted from 16-day-old chicken embryos using the method of differential adhesion. AECII were exposed to PM2.5 and TBHQ alone or in combination, and then, cell membrane integrity, pyroptosis, and necroptosis were detected. Our results showed that PM2.5 from broiler houses caused cell rupture and loss of cell membrane integrity. This result was confirmed by the obvious increases in lactate dehydrogenase (LDH) release and propidium iodide (PI)-positive cells compared to the control group. In addition, the intracellular reactive oxygen species (ROS) levels and the expression levels of pyroptosis-related genes (NLRP3, IL-18, IL-1β) and necroptosis-related genes (RIPK3) were also significantly enhanced. However, TBHQ significantly inhibited intracellular ROS, improved cell viability, and reduced the release of LDH and the number of PI-positive cells compared to those in the PM2.5 group. The expression levels of pyroptosis-related genes (Caspase-1, NLRP3, IL-18, IL-1β) and necroptosis-related genes (RIPK3) were also significantly decreased in the co-treatment group. In summary, these results indicated that TBHQ can alleviate PM2.5-mediated cell pyroptosis and necroptosis in chicken AECII and provide a basis for overcoming the danger that air pollutants from broiler houses pose to the health of chickens.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5effaa38e0a1e41569598c82468dde66Test
https://doi.org/10.1016/j.psj.2021.101593Test

المؤلفون: Bin Yin, Endong Bao, Di Wu, Shu Tang, Erbao Song, Jiao Xu

المصدر: Poultry Science. 96:1426-1437

مصطلحات موضوعية: 0301 basic medicine, Hot Temperature, Ubiquinone, Apoptosis, Creatine, 03 medical and health sciences, chemistry.chemical_compound, Western blot, Lactate dehydrogenase, medicine, Animals, Creatine Kinase, MB Form, HSP70 Heat-Shock Proteins, Myocytes, Cardiac, Cells, Cultured, chemistry.chemical_classification, L-Lactate Dehydrogenase, medicine.diagnostic_test, Caspase 3, General Medicine, Molecular biology, In vitro, Hsp70, 030104 developmental biology, Enzyme, chemistry, Quercetin, Animal Science and Zoology, Chickens, Heat-Shock Response, Immunostaining

الوصف: The aim of this study was to investigate whether induction of Hsp70 expression by co-enzyme Q10 (Q10) treatment protects chicken primary myocardial cells (CPMCs) from damage and apoptosis in response to heat stress for 5 hours. Analysis of the expression and distribution of Hsp70 and the levels of the damage-related enzymes creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH), as well as pathological analysis showed that co-enzyme Q10 alleviated the damage caused to CPMCs during heat stress. Further, analysis of cell apoptosis and the expression of cleaved caspase-3 indicated that co-enzyme Q10 did have an anti-apoptotic role during heat stress. Western blot analysis showed that pretreatment with co-enzyme Q10 led to a significant increase in the expression of Hsp70 during heat stress. Immunostaining assays confirmed the results of western blot analysis and also showed that co-enzyme Q10 could accelerate the translocation of Hsp70 into the nucleus during heat stress, but this was not observed in the group that was treated with only co-enzyme Q10. These findings seem to indicate that co-enzyme Q10 protected CPMCs from heat stress via the induction of Hsp70. To investigate this, 200 μM quercetin, an Hsp70 inhibitor, was used to inhibit the expression of Hsp70 2 h before heat stress. Quercetin pre-treatment was observed to suppress the expression of Hsp70 as well the protective function of co-enzyme Q10 at 5 h of heat stress. This finding confirms that Q10 brought about its effects via Hsp70 expression, but the mechanism underlying this needs further investigation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3914f2dc33d61b0dacca9934467bdcebTest
https://doi.org/10.3382/ps/pew402Test