Digital Quantification of Gene Expression in Sequential Breast Cancer Biopsies Reveals Activation of an Immune Response
| العنوان: | Digital Quantification of Gene Expression in Sequential Breast Cancer Biopsies Reveals Activation of an Immune Response |
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| المؤلفون: | Andrew H. Beck, Andrea L. Richardson, Laura C. Collins, Myles Brown, Housheng Hansen He, J. Dirk Iglehart, Steven E. Come, Lauren Gilmore, Shannon T. Bailey, Aquila Fatima, Gilles Buchwalter, Rinath Jeselsohn, Meredith M. Regan, Lillian Werner |
| المصدر: | PLoS ONE PLoS ONE, Vol 8, Iss 5, p e64225 (2013) |
| بيانات النشر: | Public Library of Science, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Genetic Screens, Time Factors, lcsh:Medicine, Gene Expression, Invasive Ductal Carcinoma, Bioinformatics, law.invention, 0302 clinical medicine, Randomized controlled trial, law, Molecular Cell Biology, Breast Tumors, lcsh:Science, Aged, 80 and over, 0303 health sciences, Multidisciplinary, medicine.diagnostic_test, Genomics, Middle Aged, 3. Good health, Neoplasm Proteins, Oncology, 030220 oncology & carcinogenesis, Medicine, Female, Research Article, medicine.medical_specialty, Biopsy, Fine-Needle, Breast Neoplasms, Molecular Genetics, 03 medical and health sciences, Immune system, Breast cancer, Genomic Medicine, Genome Analysis Tools, Molecular genetics, Biopsy, medicine, Genetics, Cancer Genetics, Biomarkers, Tumor, Humans, Genetic Testing, Biology, 030304 developmental biology, Genetic testing, Aged, Neoplasm Staging, business.industry, Gene Expression Profiling, lcsh:R, Computational Biology, Cancers and Neoplasms, Human Genetics, medicine.disease, Human genetics, Gene expression profiling, Genetics of Disease, lcsh:Q, Neoplasm Grading, business, Genome Expression Analysis |
| الوصف: | Advancements in molecular biology have unveiled multiple breast cancer promoting pathways and potential therapeutic targets. Large randomized clinical trials remain the ultimate means of validating therapeutic efficacy, but they require large cohorts of patients and are lengthy and costly. A useful approach is to conduct a window of opportunity study in which patients are exposed to a drug pre-surgically during the interval between the core needle biopsy and the definitive surgery. These are non-therapeutic studies and the end point is not clinical or pathological response but rather evaluation of molecular changes in the tumor specimens that can predict response. However, since the end points of the non-therapeutic studies are biologic, it is critical to first define the biologic changes that occur in the absence of treatment. In this study, we compared the molecular profiles of breast cancer tumors at the time of the diagnostic biopsy versus the definitive surgery in the absence of any intervention using the Nanostring nCounter platform. We found that while the majority of the transcripts did not vary between the two biopsies, there was evidence of activation of immune related genes in response to the first biopsy and further investigations of the immune changes after a biopsy in early breast cancer seem warranted. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad04d143678f1fcd1170bc5fe32853c8Test http://europepmc.org/articles/PMC3669373Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad04d143678f1fcd1170bc5fe32853c8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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