Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study
| العنوان: | Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study |
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| المؤلفون: | Mayfong Mayxay, Phoutthalavanh Souvannasing, Stuart D. Blacksell, Audrey Dubot-Pérès, Koukeo Phommasone, Sommay Keomany, Xavier de Lamballerie, Ooyanong Phonemixay, Manivanh Vongsouvath, Paul N. Newton, Onanong Sengvilaipaseuth |
| المصدر: | PLoS ONE, Vol 12, Iss 1, p e0170359 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Veterinary medicine, Physiology, Fevers, lcsh:Medicine, Diagnostic accuracy, Dengue virus, medicine.disease_cause, Pathology and Laboratory Medicine, Biochemistry, Dengue fever, Geographical Locations, 0302 clinical medicine, Specimen Storage, Immune Physiology, Medicine and Health Sciences, Medicine, 030212 general & internal medicine, lcsh:Science, Rapid diagnostic test, Multidisciplinary, Immune System Proteins, Follow up studies, Diagnostic test, Hematology, 3. Good health, Body Fluids, Solutions, Blood, Laos, Physical Sciences, Anatomy, Research Article, Asia, Materials by Structure, Diluents, 030231 tropical medicine, Materials Science, Immunology, Ns1 antigen, Research and Analysis Methods, Antibodies, 03 medical and health sciences, Signs and Symptoms, Diagnostic Medicine, parasitic diseases, Viral immunology, business.industry, lcsh:R, Biology and Life Sciences, Proteins, medicine.disease, Mixtures, Storage and Handling, People and Places, lcsh:Q, business |
| الوصف: | The Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after longterm storage at high temperatures. We extended this study by testing sera from 119 febrile patients collected between July-November 2012 at Salavan Provincial Hospital (southern Laos) with RDTs stored for 6 months at 4 degrees C, 35 degrees and in a hut (miniature traditional house) at Lao ambient temperatures. The dengue NS1 antigen results from RDTs stored at 35 degrees C and in the hut demonstrated 100% agreement with those stored at 4 degrees C. However, lower positive percent agreements, with broad 95% CI, were observed for the tests: IgM, 60% (14.7-94.7) and 40% (5.3-85.3) for RDTs store at 35 degrees C and in the hut, compared to those stored at 4 degrees C, respectively. This study strenghtens the evidence of the robustness of the NS1 antigen detection RDT for the diagnosis of dengue after storage at tropical temperatures. |
| وصف الملف: | text/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::981622cbedb379adca30ee5ba98ea67dTest http://europepmc.org/articles/PMC5271306?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....981622cbedb379adca30ee5ba98ea67d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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